Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7262
Title: PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania
Authors: Santos, Diego Moura
Carneiro, Marcia Weber
Moura, Tatiana Rodrigues de
Soto, Manuel
Luz, Nívea Farias
Prates, Deboraci Brito
Irache, Juan Manuel
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Espuelas, Socorro
Borges, Valeria de Matos
Oliveira, Camila Indiani de
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidad Autonoma de Madrid. Centro de Biología Molecular Severo Ochoa. Departamento de Biologia Molecular. Madrid, Spain
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidad de Navarra. Instituto de Salud Tropical. Pamplona, Spain
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brazil
Universidad de Navarra. Instituto de Salud Tropical. Pamplona, Spain / Universidad de Navarra. Facultad de Farmacia. Departamento de Farmacia y Tecnología Farmacéutica. Pamplona, Spain
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, Brazil
Abstract: We recently demonstrated that immunization with polyester poly(lactide-co-glycolide acid) (PLGA) nanoparticles loaded with the 11-kDa Leishmania vaccine candidate kinetoplastid membrane protein 11 (KMP-11) significantly reduced parasite load in vivo. Presently, we explored the ability of the recombinant PLGA nanoparticles to stimulate innate responses in macrophages and the outcome of infection with Leishmania braziliensis in vitro. Incubation of macrophages with KMP-11-loaded PLGA nanoparticles significantly decreased parasite load. In parallel, we observed the augmented production of nitric oxide, superoxide, TNF-α and IL-6. An increased release of CCL2/MCP-1 and CXCL1/KC was also observed, resulting in macrophage and neutrophil recruitment in vitro. Lastly, the incubation of macrophages with KMP-11-loaded PLGA nanoparticles triggered the activation of caspase-1 and the secretion of IL-1β and IL-18, suggesting inflammasome participation. Inhibition of caspase-1 significantly increased the parasite load. We conclude that KMP-11-loaded PLGA nanoparticles promote the killing of intracellular Leishmania parasites through the induction of potent innate responses.
Keywords: Leishmania
PLGA
Nanoparticle
Macrophage
Innate response
Issue Date: 2013
Publisher: Elsevier
Citation: SANTOS, D. M. et al. PLGA nanoparticles loaded with KMP-11 stimulate innate immunity and induce the killing of Leishmania. Nanomedicine: Nanotechnology, Biology, and Medicine, v. 9, p. 985–995, 2013.
DOI: 10.1016/j.nano.2013.04.003
ISSN: 1549-9634
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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