Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7263
Title: Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
Authors: Carregaro, Vanessa
Costa, Diego Luis
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Cunha, Fernando Queiroz
Silva, João Santana da
Affilliation: University of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brasil
University of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, Brasil / Institute Immunological Research. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, Brasil / Institute Immunological Research. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, Brasil / Institute Immunological Research. Salvador, BA, Brasil
University of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brasil / University of São Paulo. School of Medicine. Department of Pharmacology. Ribeirão Preto, SP, Brasil.
University of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brasil
Abstract: Leishmania parasites are transmitted to their vertebrate hosts by infected Phlebotomine sand flies during the blood meal of the flies. Sand fly saliva is known to enhance Leishmania spp. infection, while preexposure to saliva protects mice against parasitic infections. In this study, we investigated the initial inflammatory leucocyte composition induced by one or three inocula of salivary gland extract (SGE) from Lutzomyia longipalpis in the presence or absence of Leishmania braziliensis. Results: We demonstrated that inoculating SGE once (SGE-1X) or three times (SGE-3X), which represented a coinoculation or a pre-exposure to saliva, respectively, resulted in different cellular infiltrate profiles. Whereas SGE-1X led to the recruitment of all leucocytes subtypes including CD4+ T cells, CD4+CD25+ T cells, dendritic cells, macrophages and neutrophils, the immune cell profile in the SGE-3X group differed dramatically, as CD4+ T cells, CD4+CD25+ T cells, dendritic cells, macrophages and neutrophils were decreased and CD8+ T cells were increased. The SGE-1X group did not show differences in the ear lesion size; however, the SGE-1X group harbored a higher number of parasites. On the other hand, the SGE-3X group demonstrated a protective effect against parasitic disease, as the parasite burden was lower even in the earlier stages of the infection, a period in which the SGE-1X group presented with larger and more severe lesions. These effects were also reflected in the cytokine profiles of both groups. Whereas the SGE-1X group presented with a substantial increase in IL-10 production, the SGE-3X group showed an increase in IFN-γ production in the draining lymph nodes. Analysis of the inflammatory cell populations present within the ear lesions, the SGE-1X group showed an increase in CD4+FOXP3+ cells, whereas the CD4+FOXP3+ population was reduced in the SGE-3X group. Moreover, CD4+ T cells and CD8+ T cells producing IFN-γ were highly detected in the ears of the SGE-3X mice prior to infection. In addition, upon treatment of SGE-3X mice with anti-IFN-γ monoclonal antibody, we observed a decrease in the protective effect of SGE-3X against L. braziliensis infection. Conclusions: These results indicate that different inocula of Lutzomyia longipalpis salivary gland extract can markedly modify the cellular immune response, which is reflected in the pattern of susceptibility or resistance to Leishmania braziliensis infection.
Keywords: Phlebotomines saliva,
Lutzomyia longipalpis saliva
Leishmania braziliensis
Inflammatory leucocytes
Cytokines
Immunoregulation
Issue Date: 2013
Publisher: BioMed Central
Citation: CARREGARO, V. et al. Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear. BMC Microbiology (Online), v.13, p.102-112, 2013.
DOI: 10.1186/1471-2180-13-102
ISSN: 1471-2199
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Carregaro V Dual effect....pdf822.58 kBAdobe PDFThumbnail
View/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.