Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7267
Title: Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection
Authors: Moura, Tatiana Rodrigues de
Oliveira, Fabiano
Carneiro, Marcia Weber
Miranda, José Carlos
Clarêncio, Jorge
Barral Netto, Manoel
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Ribeiro, José Marcos Chaves
Valenzuela, Jesus G
Oliveira, Camila Indiani de
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
National Institute of Allergy and Infectious Diseases. National Institutes of Health. Laboratory of Malaria and Vector Research. Rockville, Maryland, United States of America
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, Bahia, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, Bahia, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, Bahia, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, Bahia, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, Bahia, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, Bahia, Brazil
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research.Rockville, Maryland, United States of America
National Institute of Allergy and Infectious Diseases. National Institutes of Health. Laboratory of Malaria and Vector Research. Rockville, Maryland, United States of America
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, BA, Brazil,
Abstract: Background: Leishmania parasites are transmitted in the presence of sand fly saliva. Together with the parasite, the sand fly injects salivary components that change the environment at the feeding site. Mice immunized with Phlebotomus papatasi salivary gland (SG) homogenate are protected against Leishmania major infection, while immunity to Lutzomyia intermedia SG homogenate exacerbated experimental Leishmania braziliensis infection. In humans, antibodies to Lu. intermedia saliva are associated with risk of acquiring L. braziliensis infection. Despite these important findings, there is no information regarding the repertoire of Lu. intermedia salivary proteins. Methods and Findings: A cDNA library from the Salivary Glands (SGs) of wild-caught Lu. intermedia was constructed, sequenced, and complemented by a proteomic approach based on 1D SDS PAGE and mass/mass spectrometry to validate the transcripts present in this cDNA library. We identified the most abundant transcripts and proteins reported in other sand fly species as well as novel proteins such as neurotoxin-like proteins, peptides with ML domain, and three small peptides found so far only in this sand fly species. DNA plasmids coding for ten selected transcripts were constructed and used to immunize BALB/c mice to study their immunogenicity. Plasmid Linb-11—coding for a 4.5-kDa protein—induced a cellular immune response and conferred protection against L. braziliensis infection. This protection correlated with a decreased parasite load and an increased frequency of IFN-c-producing cells. Conclusions: We identified the most abundant and novel proteins present in the SGs of Lu. intermedia, a vector of cutaneous leishmaniasis in the Americas. We also show for the first time that immunity to a single salivary protein from Lu. intermedia can protect against cutaneous leishmaniasis caused by L. braziliensis.
DeCS: Leishmania
Leishmania braziliensis
Lutzomyia
Glândulas salivares
Infecção
SAliva
Issue Date: 2013
Publisher: Public Library of Science
Citation: MOURA, T. R. de et al. Functional transcriptomics of wild-caught Lutzomyia intermedia salivary glands: identification of a protective Salivary protein against Leishmania braziliensis infection. PLoS Neglected Tropical Diseases (Online), v.7, n. 5, p. e2242-e2257, 2013.
DOI: 10.1371/journal.pntd.0002242
ISSN: 1935-2727
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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