Mostrar el registro sencillo del ítem

AutorRocha, Roberta Dias Rodrigues
AutorGontijo, Célia Maria Ferreira
AutorSantos, Silvana Maria Elói
AutorCarvalho, Andréa Teixeira de
AutorOliveira, Rodrigo Corrêa de
AutorFerrari, Teresa Cristina de Abreu
AutorMarques, Marcos José
AutorMayrink, Wilson
AutorMartins Filho, Olindo Assis
Fecha de acceso2013-12-18T17:04:01Z
Fecha de disponibilización2013-12-18T17:04:01Z
Fecha de publicación2013
Referencia101- ROCHA, Roberta Dias Rodrigues et al. Clinical value of anti-live Leishmania (Viannia) braziliensis immunoglobulin G subclasses, detected by flow cytometry, for diagnosing active localized cutaneous leishmaniasis. Trop Med Int Health. 2006 Feb;11(2):156-66.pt_BR
ISSN1360-2276
URIhttps://www.arca.fiocruz.br/handle/icict/7281
Idiomaporpt_BR
EditorBlackwell Scientific Publicationspt_BR
Derechos de autoropen accesspt_BR
TítuloClinical value of anti-live Leishmania (Viannia) braziliensis immunoglobulin G subclasses, detected by flow cytometry, for diagnosing active localized cutaneous leishmaniasispt_BR
Tipo del documentoArticlept_BR
Resumen en InglésOBJECTIVE: To evaluate the clinical value of flow cytometry anti-live promastigate antibody (FC-ALPA), for diagnosing active cutaneous leishmaniasis. METHOD: Serum samples from 145 individuals living in endemic areas for localized cutaneous leishmaniasis (population 1) were classified as having the disease or not and then tested for their IgG reactivity by indirect immunofluorescence assay and FC-ALPA-IgG. The results of FC-ALPA-IgG were expressed as percentage of positive fluorescent parasite. Both tests were also evaluated in serum samples of people with visceral leishmaniasis and Chagas disease (population 1A). RESULTS: In population 1, FC-ALPA-IgG performed better than the immunofluorescence assay regarding sensitivity, specificity and predictive values. Analysis of the results according to the likelihood ratios indicated that a percentage of positive fluorescent parasite <or=60 practically excludes the diagnosis of localized cutaneous leishmaniasis (likelihood ratio=0.07), whereas at >60% it reinforces diagnosis of the disease (likelihood ratio = 7.0). Immunofluorescent assay is of little value (likelihood ratio=2.04). In population 1A, both tests performed worse, but FC-ALPA-IgG achieved better statistical indexes than immunofluorescent assay. CONCLUSION: The FC-ALPA-IgG is a valuable method for serological diagnosis of localized cutaneous leishmaniasis. FC-ALPA-IgG1/ALPA-IgG2 combined analysis is an additional serological tool for discriminating localized visceral leishmaniasis, Chagas disease and visceral leishmaniasis in areas where these infections co-exist.pt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Leishmanioses. Belo Horizonte, MG, Brazilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazil/Universidade Federal de Ouro Preto. Escola de Farmacia. Ouro Preto, MG, Brazilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazilpt_BR
AfiliaciónUniversidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazilpt_BR
AfiliaciónUniversidade Federal de Juiz de Fora. Laboratorio de Imunologia. Juiz de Fora, MG, Brazilpt_BR
AfiliaciónUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Doença de Chagas. Belo Horizonte, MG, Brazilpt_BR
Palavras clave en Inglêslocalized cutaneous leishmaniasispt_BR
Palavras clave en Inglêsanti-live Leishmania (V.)pt_BR
Palavras clave en Inglêsbraziliensis promastigotespt_BR
Palavras clave en InglêsIgG antibodiespt_BR
Palavras clave en Inglêsserodiagnosispt_BR
Palavras clave en Inglêsflow cytometrypt_BR
Palavras clave en Inglêsperformance indexespt_BR


Ficheros en el ítem

Nombre:
101.pdf
Tamaño:
1.116Mb
Formato:
application/
Ver/Abrir

Este ítem aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem