Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7283
Title: Cytotoxic, immunosuppressive and trypanocidal activities of agrocybin, a polyacetylene produced by Agrocybe perfecta (Basidiomycota).
Authors: Rosa, Luiz Henrique
Fagundes, Elaine Maria de Souza
Machado, Kátia Maria Gomes
Alves, Tânia Maria de Almeida
Martins Filho, Olindo Assis
Romanha, Álvaro José
Oliveira, Rodrigo Corrêa de
Rosa, Carlos Augusto
Zani, Carlos Leomar
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais Instituto de Ciencias Biologicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brasil
Fundação Centro Tecnológico de Minas Gerais. Belo Horizonte, MG, Brasil/Universidade Católica de Santos. Santos, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Doenças de Chagas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de imunologia. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brasil
Abstract: The ethyl acetate extract from the culture of the fungusAgrocybe perfecta(Rick) Singer was selected for further study in a screening of Brazilian basidiomycetes for bioactivity. The extract showed significant activity against the recombinant enzyme trypanothione reductase (TryR) fromTrypanosoma cruzi, lymphocyte proliferation in human peripheral mononuclear cells (PBMC) stimulated with phytohemaglutinin (PHA), and the human cancer cell lines UACC-62 (melanoma), MCF-7 (mammary), and TK-10 (kidney). The chromatographic fractionation of the extract was monitored by the above bioassays and showed that agrocybin was the active component. Agrocybin, a known polyacetylene amide, showed an IC50of 2lM in the TryR assay but killed only 60% of the trypomastigote form of T. cruzi in infected murine blood even at 680 lM. This weaker activity could be due to the low temperature used to mimic banked blood or as a consequence of its inactivation by blood, already reported in the literature. On the other hand, it inhibited the proliferation of PBMC by 50% at 3.4lM and the growth of the cancer cell lines at concentrations between 9 and 24.5lM. Measurements of DNA fragmentation using flow cytometry suggest that agrocybin promotes cell death via apoptosis.
Keywords: Basidiomycetes
cancer cells
lymphocytes
PBMC
trypanothione reductase
Trypanosoma cruzi
Issue Date: 2006
Publisher: Springer
Citation: ROSA, Luiz Henrique et al. Cytotoxic, immunosuppressive and trypanocidal activities of agrocybin, a polyacetylene produced by Agrocybe perfecta (Basidiomycota). World Journal of Microbiology and Biotechnology. 2006; Volume 22(6): 539-545
DOI: 10.1007/s11274-005-9002-7
ISSN: 0959-3993
Copyright: restricted access
Appears in Collections:MG - IRR - Artigos de Periódicos

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