Author | Silva, Robson Amaro Augusto da | |
Author | Tavares, Natalia Machado | |
Author | Costa, Dirceu Joaquim | |
Author | Pitombo, Maiana A | |
Author | Barbosa, Larissa | |
Author | Fukutani, Kiyoshi Ferreira | |
Author | Miranda, José Carlos | |
Author | Oliveira, Camila Indiani de | |
Author | Valenzuela, Jesus G | |
Author | Barral, Aldina Maria Prado | |
Author | Soto, Manuel | |
Author | Barral Netto, Manoel | |
Author | Brodskyn, Claudia Ida | |
Access date | 2014-02-10T14:08:38Z | |
Available date | 2014-02-10T14:08:38Z | |
Document date | 2011 | |
Citation | SILVA, R. R. A. da et al. DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis. Acta Tropica, v. 120, n. 3, p. 185-190, 2011. | pt_BR |
ISSN | 0001-706X | |
URI | https://www.arca.fiocruz.br/handle/icict/7306 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | open access | pt_BR |
Title | DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.actatropica.2011.08.007 | |
Abstract | It was recently shown that immunization of hamsters with DNA plasmids coding LJM19, a sand fly salivary protein, partially protected against a challenge with Leishmania chagasi, whereas immunization with KMP11 DNA plasmid, a Leishmania antigen, induced protection against L. donovani infection. In the present study, we evaluated the protective effect of immunization with both LJM19 and KMP11 DNA plasmid together. Concerning the protection against an infection by L. chagasi, immunization with DNA plasmids coding LJM19 or KMP11, as well as with both plasmids combined, induced IFN-γ production in draining lymph nodes at 7, 14 and 21 days post-immunization. Immunized hamsters challenged with L. chagasi plus Salivary Gland Sonicate (SGS) from Lutzomyia longipalpis showed an enhancement of IFN-γ/IL-10 and IFN-γ/TGF-ß in draining lymph nodes after 7 and 14 days of infection. Two and five months after challenge, immunized animals showed reduced parasite load in the liver and spleen, as well as increased IFN-γ/IL-10 and IFN-γ/TGF-ß ratios in the spleen. Furthermore, immunized animals remained with a normal hematological profile even five months after the challenge, whereas L. chagasi in unimmunized hamsters lead to a significant anemia. The protection observed with LJM19 or KMP11 DNA plasmids used alone was very similar to the protection obtained by the combination of both plasmids | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Vitória da Conquista, BA, Brasil / Faculdade de Medicina. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. United States of America | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidad Autónoma de Madrid. Departamento de Biologia Molecular. Centro de Biologia Molecular Severo Ochoa. Madrid, Spain | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. (iii-INCT). São Paulo, SP, Brasil | pt_BR |
Subject | Hamster | pt_BR |
Subject | Leishmania chagasi | pt_BR |
Subject | Visceral leishmaniasis | pt_BR |
Subject | Saliva | pt_BR |
Subject | DNA plasmids | pt_BR |
Subject | Protection | pt_BR |
DeCS | Proteínas de Insetos/imunologia | pt_BR |
DeCS | Leishmaniose Visceral/prevenção & controle | pt_BR |
DeCS | Glicoproteínas de Membrana/imunologia | pt_BR |
DeCS | Proteínas de Protozoários/imunologia | pt_BR |
DeCS | Psychodidae/imunologia | pt_BR |
DeCS | Proteínas e Peptídeos Salivares/imunologia | pt_BR |
DeCS | Vacinas de DNA/imunologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Cricetinae | pt_BR |
DeCS | Feminino | pt_BR |
DeCS | Proteínas de Insetos/genética | pt_BR |
DeCS | Interferon gama/secreção | pt_BR |
DeCS | Interleucina-10/secreção | pt_BR |
DeCS | Leishmaniose Visceral/imunologia | pt_BR |
DeCS | Fígado/parasitologia | pt_BR |
DeCS | Linfonodos/imunologia | pt_BR |
DeCS | Masculino | pt_BR |
DeCS | Glicoproteínas de Membrana/genética | pt_BR |
DeCS | Mesocricetus | pt_BR |
DeCS | Proteínas Recombinantes/administração & dosagem | pt_BR |
DeCS | Baço/imunologia | pt_BR |
DeCS | Baço/parasitologia | pt_BR |
DeCS | Fator de Crescimento Transformador beta/secreção | pt_BR |