Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7306
Title: DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis
Authors: Silva, Robson Amaro Augusto da
Tavares, Natalia Machado
Costa, Dirceu Joaquim
Pitombo, Maiana A
Barbosa, Larissa
Fukutani, Kiyoshi Ferreira
Miranda, José Carlos
Oliveira, Camila Indiani de
Valenzuela, Jesus G
Barral, Aldina Maria Prado
Soto, Manuel
Barral Netto, Manoel
Brodskyn, Claudia Ida
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Vitória da Conquista, BA, Brasil / Faculdade de Medicina. Instituto de Ciências da Saúde. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. United States of America
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidad Autónoma de Madrid. Departamento de Biologia Molecular. Centro de Biologia Molecular Severo Ochoa. Madrid, Spain
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. (iii-INCT). São Paulo, SP, Brasil
Abstract: It was recently shown that immunization of hamsters with DNA plasmids coding LJM19, a sand fly salivary protein, partially protected against a challenge with Leishmania chagasi, whereas immunization with KMP11 DNA plasmid, a Leishmania antigen, induced protection against L. donovani infection. In the present study, we evaluated the protective effect of immunization with both LJM19 and KMP11 DNA plasmid together. Concerning the protection against an infection by L. chagasi, immunization with DNA plasmids coding LJM19 or KMP11, as well as with both plasmids combined, induced IFN-γ production in draining lymph nodes at 7, 14 and 21 days post-immunization. Immunized hamsters challenged with L. chagasi plus Salivary Gland Sonicate (SGS) from Lutzomyia longipalpis showed an enhancement of IFN-γ/IL-10 and IFN-γ/TGF-ß in draining lymph nodes after 7 and 14 days of infection. Two and five months after challenge, immunized animals showed reduced parasite load in the liver and spleen, as well as increased IFN-γ/IL-10 and IFN-γ/TGF-ß ratios in the spleen. Furthermore, immunized animals remained with a normal hematological profile even five months after the challenge, whereas L. chagasi in unimmunized hamsters lead to a significant anemia. The protection observed with LJM19 or KMP11 DNA plasmids used alone was very similar to the protection obtained by the combination of both plasmids
Keywords: Hamster
Leishmania chagasi
Visceral leishmaniasis
Saliva
DNA plasmids
Protection
DeCS: Proteínas de Insetos/imunologia
Leishmaniose Visceral/prevenção & controle
Glicoproteínas de Membrana/imunologia
Proteínas de Protozoários/imunologia
Psychodidae/imunologia
Proteínas e Peptídeos Salivares/imunologia
Vacinas de DNA/imunologia
Animais
Cricetinae
Feminino
Proteínas de Insetos/genética
Interferon gama/secreção
Interleucina-10/secreção
Leishmaniose Visceral/imunologia
Fígado/parasitologia
Linfonodos/imunologia
Masculino
Glicoproteínas de Membrana/genética
Mesocricetus
Proteínas Recombinantes/administração & dosagem
Baço/imunologia
Baço/parasitologia
Fator de Crescimento Transformador beta/secreção
Issue Date: 2011
Publisher: Elsevier
Citation: SILVA, R. R. A. da et al. DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis. Acta Tropica, v. 120, n. 3, p. 185-190, 2011.
DOI: 10.1016/j.actatropica.2011.08.007
ISSN: 0001-706X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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