Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7309
Title: TNF-alpha and IL-8: serum levels and gene polymorphisms (-308G>A and -251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia
Authors: Cajado, Cyntia
Cerqueira, Bruno Antonio Veloso
Couto, Fábio David
Santos, Wendell Vilas Boas
Dorea, M. J
Lyra, Isa Menezes
Barbosa, Cynara Gomes
Reis, Mitermayer Galvão dos
Gonçalves, Marilda de Souza
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação de Hematologia e Hemoterapia do Estado da Bahia. HEMOBA. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil
Abstract: Sickle cell anemia (SCA) is a disorder characterized by a heterogeneous clinical outcome. In the present study, we investigated the associations between Tumor Necrosis Factor-alpha (TNF-alpha) 308G>A and Interleukin 8 (IL-8) 251A>T gene polymorphisms, medical history and classical biomarkers in children with steady-state SCA. In total, 210 SCA patients aged 2–21 years and 200 healthy controls were studied. Gene polymorphisms, betaS-globin haplotypes and a 3.7-kb deletion in alpha2-thalassemia (a2-thal3.7 kb) were investigated by PCR/RFLP analysis, and cytokine levels were determined by ELISA. Splenomegaly (p = .032) was more prevalent among children younger than 5 years of age. The A allele of the TNF-alpha 308G>A gene polymorphism and the presence of a2-thal3.7 kb were associated with an increase risk of splenic sequestration events (p = .001; p = .046), while the T allele of the IL-8 251A>T gene polymorphism was considered to be a protective factor for splenomegaly events (p = .032). Moreover, the A allele of the TNF-alpha 308G>A gene polymorphism was associated with high TNF-alpha levels (p = .021), and the hemoglobin F and hemoglobin S haplotypes were correlated with serum levels of IL-8. The logistic regression analysis showed significant effects of the TNF-alpha and IL-8 gene polymorphisms, betaS-globin gene haplotypes and a2-thal3.7 kb on the occurrence of splenic sequestration events. Our study emphasizes that the identification of new genetic and immunological biomarkers and their associations with classical markers is an important strategy to elucidate the underlying causes of different SCA phenotypes and their effects on patient outcome
Keywords: Sickle cell anemia
TNF-alpha
IL-8
betaS-globin Gene haplotypes
alpha2-Thalassemia
DeCS: Anemia Falciforme/sangue
Marcadores Biológicos/sangue
Interleucina-8/sangue
Anamnese
Polimorfismo Genético
Fator de Necrose Tumoral alfa/sangue
Adolescente
Adulto
Alelos
Anemia Falciforme/genética
Estudos de Casos e Controles
Criança
Pré-Escolar
Estudos Transversais
Ensaio de Imunoadsorção Enzimática
Haplotipos
Humanos
Interleucina-8/genética
Reação em Cadeia da Polimerase
Polimorfismo de Fragmento de Restrição
Fator de Necrose Tumoral alfa/genética
Issue Date: 2011
Publisher: Elsevier
Citation: CAJADO, C. et al. TNF-alpha and IL-8: serum levels and gene polymorphisms (-308G>A and -251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia. Cytokine, v. 56, n. 2, p. 312-317, 2011.
DOI: 10.1016/j.cyto.2011.07.002
ISSN: 1043-4666
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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