Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7320
Title: Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP
Authors: Grassi, Maria Fernanda Rios
Gallazzi, Viviana Nilla Olavarria
Kruschewsky, Ramon de Almeida
Mascarenhas, Rita Elizabeth Moreira
Dourado, Inês
Correia, Luis Cláudio Lemos
Costa, Carlos Maurício de Castro
Castro Filho, Bernardo Galvão
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, Brasil
Bahian School of Medicine and Public Health. EBMSP. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, Brasil
Federal University of Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil
Bahian School of Medicine and Public Health. EBMSP. Salvador, BA, Brasil
Federal University of Ceará. Laboratory of Experimental Neurology and Neurophysiology. Fortaleza, CE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Advanced Laboratory of Public Health. Salvador, BA, Brasil
Abstract: A high human T-cell lymphotropic virus type 1 (HTLV-1) proviral load is described in HTLV-1-associated diseases, especially HAM/TSP. However, the cut-off value to define high levels of HTLV-1 proviral load is not well established. 281 HTLV-1-infected patients from the HTLV reference center in Salvador, Brazil, were followed from 2005 to 2008. Patients were classified as asymptomatic, possible-, probable-, and definite-HAM/TSP, in accordance with diagnostic criteria proposed by De Castro-Costa et al. (2006): AIDS Res Hum Retroviruses 22:931-935. HTLV-1 proviral load was determined using real-time PCR. A receiver operator characteristic (ROC) curve was constructed using only asymptomatic individuals and definite-HAM/TSP patients. The ROC curve was used to predict the proviral load level that differentiates these two groups. Out of 281 patients, 189 were asymptomatic and 92 were diagnosed with HAM/TSP (22 possible, 23 probable, 47 definite). The mean HTLV-1 proviral load was higher in possible- (89,104 ± 93,006 copies/106 PBMC), -probable (175,854 ± 128,083 copies/106 PBMC), and definite-HAM/TSP patients (150,667 ± 122,320 copies/106 PBMC),when compared to asymptomatic individuals (27,178 ± 41,155 copies/106 PBMC) (P < 0.0001). A comparison of all HAM/TSP groups showed the highest proviral loads in probable-HAM/TSP patients, yet the differences in mean values were not statistically significant. The ROC curve suggested a value of 49,865 copies/106 PBMC, with 87% sensitivity (95% CI » 74-95) and 81% specificity (95% CI » 75-86), as the best proviral load cut-off point to differentiate definite HAM/TSP patients from asymptomatic individuals. HTLV-1 proviral loads are higher in groups of infected patients with eurological symptoms and may represent a relevant biological marker of disease progression.
Keywords: HTLV-1
Proviral load
Biomarker
HAM/TSP
Brazil
DeCS: Infecções por HTLV-I/diagnóstico
Infecções por HTLV-I/virologia
Vírus 1 Linfotrópico T Humano/crescimento & desenvolvimento
Paraparesia Espástica Tropical/diagnóstico
Paraparesia Espástica Tropical/virologia
Provirus/crescimento & desenvolvimento
Doenças da Medula Espinal/diagnóstico
Doenças da Medula Espinal/virologia
Carga Viral
Adolescente
Adulto
Idoso
Idoso de 80 Anos ou mais
Doenças Assintomáticas
Marcadores Biológicos
Brasil
Criança
DNA Viral/análise
Progressão da Doença
Feminino
Infecções por HTLV-I/complicações
Infecções por HTLV-I/patologia
Vírus 1 Linfotrópico T Humano/genética
Leucócitos Mononucleares/patologia
Humanos
Paraparesia Espástica Tropical/complicações
Reação em Cadeia da Polimerase
Valor Preditivo dos Testes
Provirus/genética
Doenças da Medula Espinal/complicações
Doenças da Medula Espinal/patologia
Issue Date: 2011
Publisher: Wiley-Liss, INC
Citation: GRASSI M. F. et al. Human T cell lymphotropic virus type 1 (HTLV-1) proviral load of HTLV-associated myelopathy/tropical spastic paraparesis (HAM/TSP) patients according to new diagnostic criteria of HAM/TSP. Journal of Medical Virology, v. 83, n. 7, p. 1269-1274, 2011.
DOI: 10.1002/jmv.22087
ISSN: 1096-9071
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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