Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7356
Title: TGFB1 and IL8 gene polymorphisms and susceptibility to visceral leishmaniasis
Authors: Frade, Amanda Farage
Oliveira, Lea Campos de
Costa, Dorcas Lamounier
Costa, Carlos Henrique Nery
Aquino, Dorlene Maria Cardoso de
Van Weyenbergh, Johan Jozef Rosa Maria
Barral Netto, Manoel
Barral, Aldina Maria Prado
Kalil, Jorge
Goldberg, Anna Carla
Affilliation: Heart Institute (InCor) HC- FMUSP. Laboratory of Immunology. São Paulo, SP, Brasil
University of São Paulo. Central Institute. General Hospital. School of Medicine. Laboratory of Medicine Laboratorial. São Paulo, SP, Brasil
University Federal of Piauí. Teresina, PI, Brasil
University Federal of Piauí. Teresina, PI, Brasil
University Federal of Maranhão. São Luís, MA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute for Investigation in Immunology (iii)–INCT-CNPq – Brasil. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute for Investigation in Immunology (iii)–INCT-CNPq – Brasil. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Albert Einstein Institute for Education and Research. São Paulo, SP, Brasil / Institute for Investigation in Immunology (iii)–INCT-CNPq – Brasil. Brasil
Heart Institute (InCor) HC- FMUSP. Laboratory of Immunology. São Paulo, SP, Brasil / Institute for Investigation in Immunology (iii) – INCT-CNPq – Brasil. Brasil
Albert Einstein Institute for Education and Research. São Paulo, SP, Brasil / Institute for Investigation in Immunology (iii) – INCT-CNPq – Brasil. Brasil
Abstract: Visceral leishmaniasis (VL) or Kala-azar is a serious protozoan infectious disease caused by an obligate intracellular parasite. Cytokines have a major role in determining progression and severity of clinical manifestations in VL. We investigated polymorphisms in the TGFB1and IL8 genes, which are cytokines known to have a role in onset and severity of the disease. Polymorphisms at TGFB1 -509 C/T and +869 T/C, and IL8 -251 A/T were analyzed by a PCR-RFLP technique, in 198 patients with VL, 98 individuals with asymptomatic infection positive for a delayed-type hypersensitivity test (DTH+) and in 101 individuals with no evidence of infection (DTH-). The presence of the T allele in position -509 of the TGFB1 gene conferred a two-fold risk to develop infection both when including those with clinical symptoms (DTH+ and VL, grouped) or when considering DTH+ only, respectively p = 0.007, OR = 1.9 [1.19-3.02] and p = 0.012, OR = 2.01 [1.17-3.79], when compared with DTH- individuals. In addition, occurrence of hemorrhage was associated with TGFB1 -509 T allele. We suggest that the -509 T allele of the TGFB1 gene, a cytokine with a biologically relevant role in the natural history of the disease, may contribute to overall susceptibility to infection by Leishmania and to severity of the clinical disease.
Keywords: Visceral leishmaniasis
Cytokines
Gene polymorphisms
Disease susceptibility
DeCS: Predisposição Genética para Doença
Interleucina-8/genética
Leishmaniose Visceral/genética
Fator de Crescimento Transformador beta1/genética
Adolescente
Idoso
Adulto
Alelos
Criança
Pré-Escolar
Feminino
Genótipo
Humanos
Lactente
Masculino
Meia-Idade
Issue Date: 2011
Publisher: Elsevier B.V
Citation: FRADE, A. F. et al. TGFB1 and IL8 gene polymorphisms and susceptibility to visceral leishmaniasis. Infection, Genetics and Evolution, v. 11, p. 912–916, 2011.
DOI: 10.1016/j.meegid.2011.02.014
ISSN: 1567-7257
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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