Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7378
Title: Plasma superoxide dismutase-1 as a surrogate marker of vivax malaria severity
Authors: Andrade, Bruno de Bezerril
Reis Filho, Antonio José Souza
Souza Neto, Sebastião Martins
Raffaele Netto, Imbroinise
Camargo, Luís Marcelo Aranha
Barral, Aldina Maria Prado
Barral Netto, Manoel
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Universidade de São Paulo. Instituto de Ciências Biológicas. Departamento de Parasitologia. São Paulo, SP, Brasil / Faculdade de Medicina. Faculdade São Lucas. Porto Velho, RO, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia (INCT). Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia (INCT). Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Abstract: Severe outcomes have been described for both Plasmodium falciparum and P. vivax infections. The identification of sensitive and reliable markers of disease severity is fundamental to improving patient care. An intense pro-inflammatory response with oxidative stress and production of reactive oxygen species is present in malaria. Inflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) and antioxidant agents such as superoxide dismutase-1 (SOD-1) are likely candidate biomarkers for disease severity. Here we tested whether plasma levels of SOD-1 could serve as a biomarker of severe vivax malaria. METHODOLOGY/PRINCIPAL FINDINGS: Plasma samples were obtained from residents of the Brazilian Amazon with a high risk for P. vivax transmission. Malaria diagnosis was made by both microscopy and nested PCR. A total of 219 individuals were enrolled: non-infected volunteers (n = 90) and individuals with vivax malaria: asymptomatic (n = 60), mild (n = 50) and severe infection (n = 19). SOD-1 was directly associated with parasitaemia, plasma creatinine and alanine amino-transaminase levels, while TNF-alpha correlated only with the later enzyme. The predictive power of SOD-1 and TNF-alpha levels was compared. SOD-1 protein levels were more effective at predicting vivax malaria severity than TNF-alpha. For discrimination of mild infection, elevated SOD-1 levels showed greater sensitivity than TNF-alpha (76% vs. 30% respectively; p<0.0001), with higher specificity (100% vs. 97%; p<0.0001). In predicting severe vivax malaria, SOD-1 levels exhibited higher sensitivity than TNF-alpha (80% vs. 56%, respectively; p<0.0001; likelihood ratio: 7.45 vs. 3.14; p<0.0001). Neither SOD-1 nor TNF-alpha could discriminate P. vivax infections from those caused by P. falciparum. CONCLUSION: SOD-1 is a powerful predictor of disease severity in individuals with different clinical presentations of vivax malaria.
DeCS: Malária Vivax/diagnóstico
Malária Vivax/patologia
Superóxido Dismutase/sangue
Adolescente
Adulto
Animais
Marcadores Biológicos
Brasil
Feminino
Humanos
Masculino
Microscopia
Meia-Idade
Parasitemia
Plasma/química
Plasmodium vivax/imunologia
Plasmodium vivax/isolamento & purificação
Reação em Cadeia da Polimerase
Valor Preditivo dos Testes
Prognóstico
Adulto Jovem
Issue Date: 2010
Publisher: New York University School of Medicine
Citation: ANDRADE, B. B. et al. Plasma superoxide dismutase-1 as a surrogate marker of vivax malaria severity. Plos Neglected Tropical Diseases, v. 4, p. e650, 2010.
ISSN: 1935-2735
10.1371/journal.pntd.0000650
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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