Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7388
Title: Chemokines and chemokine receptors coordinate the inflammatory immune response in human cutaneous leishmaniasis
Authors: Campanelli, Ana Paula
Brodskyn, Claudia Ida
Oliveira, Viviane Sampaio Boaventura de
Silva, Claire
Roselino, Ana M
Costa, Jackson Mauricio Lopes
Saldanha, Ana Cristina Rodrigues
Freitas, Luiz Antonio Rodrigues de
Oliveira, Camila Indiani de
Barral Netto, Manoel
Silva, João Santana da
Barral, Aldina Maria Prado
Affilliation: University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology. São Paulo, SP, Brasil / University of São Paulo. Bauru Dental School. Department of Biological Sciences. São Paulo, SP. Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia (INCT). Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
University of São Paulo. School of Medicine. Division of Dermatology. Ribeirão Preto, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciéncia e Tecnologia (INCT). Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
University of São Paulo. School of Medicine of Ribeirão Preto. Department of Biochemistry and Immunology. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Instituto Nacional de Ciéncia e Tecnologia (INCT). Instituto de Investigação em Imunologia. São Paulo, SP, Brasil
Abstract: Cutaneous leishmaniasis (CL) includes different clinical manifestations displaying diverse intensities of dermal inflammatory infiltrate. Diffuse CL (DCL) cases are hyporesponsive, and lesions show very few lymphocytes and a predominance of macrophages. In contrast, localized CL (LCL) cases are responsive to leishmanial antigen, and lesions exhibit granulocytes and mononuclear cell infiltration in the early phases, changing to a pattern with numerous lymphocytes and macrophages later in the lesion. Therefore, different chemokines may affect the predominance of cell infiltration in distinct clinical manifestations. In lesions from LCL patients, we examined by flow cytometry the presence of different chemokines and their receptors in T cells, and we verified a higher expression of CXCR3 in the early stages of LCL (less than 30 days of infection) and a higher expression of CCR4 in the late stages of disease (more than 60 days of infection). We also observed a higher frequency of T cells producing IL-10 in the late stage of LCL. Using immunohistochemistry, we observed a higher expression of CCL7, CCL17 in lesions from late LCL, as well as CCR4 suggesting a preferential recruitment of regulatory T cells in the late LCL. Comparing lesions from LCL and DCL patients, we observed a higher frequency of CCL7 in DCL lesions. These results point out the importance of the chemokines, defining the different types of cells recruited to the site of the infection, which could be related to the outcome of infection as well as the clinical form observed.
Keywords: Chemokines
Cutaneous leishmaniasis
Inflammation
Receptores de Quimiocinas/imunologia
DeCS: Quimiocinas/imunologia
Inflamação/imunologia
Leishmaniose Cutânea/imunologia
Leishmaniose Cutânea/patologia
Linfócitos T/metabolismo
Adolescente
Adulto
Quimiocinas/metabolismo
Criança
Feminino
Citometria de Fluxo
Humanos
Imuno-Histoquímica
Leishmaniose Tegumentar Difusa/imunologia
Leishmaniose Tegumentar Difusa/patologia
Meia-Idade
Masculino
Receptores de Quimiocinas/metabolismo
Linfócitos T/imunologia
Adulto Jovem
Issue Date: 2010
Publisher: Elsevier
Citation: CAMPANELLI, A. P. et al. Chemokines and chemokine receptors coordinate the inflammatory immune response in human cutaneous leishmaniasis. Human Immunology, v. 71, n. 12, p. 1220-1227, 2010.
DOI: 10.1016/j.humimm.2010.09.002
ISSN: 1879-1166
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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