Author | Afonso, Lilian Maria | |
Author | Borges, Valeria de Matos | |
Author | Cruz, Heloísa | |
Author | Gomes, Flávia Lima Ribeiro | |
Author | Reis, George A. dos | |
Author | Dutra, Alberto Augusto Noronha | |
Author | Clarêncio, Jorge | |
Author | Oliveira, Camila Indiani de | |
Author | Barral, Aldina Maria Prado | |
Author | Barral Netto, Manoel | |
Author | Brodskyn, Claudia Ida | |
Access date | 2014-03-13T17:49:29Z | |
Available date | 2014-03-13T17:49:29Z | |
Document date | 2008 | |
Citation | AFONSO, L. Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis. Journal of Leukocyte Biology, v.84, n. 2, p.389-392, 2008. | pt_BR |
ISSN | 0741-5400 | |
URI | https://www.arca.fiocruz.br/handle/icict/7405 | |
Language | eng | pt_BR |
Publisher | Society for Leukocyte Biology | pt_BR |
Rights | open access | pt_BR |
Title | Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1189/jlb.0108018 | |
Abstract | Neutrophils are involved in the initial steps of most responses to pathogens. In the present study, we evaluated the effects of the interaction of apoptotic vs. necrotic human neutrophils on macrophage infection by Leishmania amazonensis. Phagocytosis of apoptotic, but not viable, neutrophils by Leishmania-infected macrophages led to an increase in parasite burden via a mechanism dependent on TGF-beta1 and PGE2. Conversely, infected macrophages' uptake of necrotic neutrophils induced killing of L. amazonensis. Leishmanicidal activity was dependent on TNF-alpha and neutrophilic elastase. Nitric oxide was not involved in the killing of parasites, but the interaction of necrotic neutrophils with infected macrophages resulted in high superoxide production, a process reversed by catalase, an inhibitor of reactive oxygen intermediate production. Initial events after Leishmania infection involve interactions with neutrophils; we demonstrate that phagocytosis of these cells in an apoptotic or necrotic stage can influence the outcome of infection, driving either parasite survival or destruction. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil | pt_BR |
Subject | Apoptosis | pt_BR |
Subject | Necrosis | pt_BR |
Subject | Phagocytosis | pt_BR |
Subject | TGF- B1 | pt_BR |
Subject | NE | pt_BR |
DeCS | Leishmaniose Cutânea/fisiopatologia | pt_BR |
DeCS | Macrófagos/parasitologia | pt_BR |
DeCS | Neutrófilos/imunologia | pt_BR |
DeCS | Neutrófilos/fisiologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Apoptose | pt_BR |
DeCS | Catalase/farmacologia | pt_BR |
DeCS | Efeitos Psicossociais da Doença | pt_BR |
DeCS | Dinoprostona/fisiologia | pt_BR |
DeCS | Humanos | pt_BR |
DeCS | Leishmania mexicana/patogenicidade | pt_BR |
DeCS | Leishmania mexicana/fisiologia | pt_BR |
DeCS | Leishmaniose Cutânea/patologia | pt_BR |
DeCS | Necrose | pt_BR |
DeCS | Neutrófilos/patologia | pt_BR |
DeCS | Fagocitose | pt_BR |
DeCS | Superóxidos/metabolismo | pt_BR |
DeCS | Fator de Crescimento Transformador beta1/fisiologia | pt_BR |