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INTERACTIONS WITH APOPTOTIC BUT NOT WITH NECROTIC NEUTROPHILS INCREASE PARASITE BURDEN IN HUMAN MACROPHAGES INFECTED WITH LEISHMANIA AMAZONENSIS
Afonso, Lilian Maria et al. | Date Issued: 2008
Author
Afonso, Lilian Maria
Borges, Valeria de Matos
Cruz, Heloísa
Gomes, Flávia Lima Ribeiro
Reis, George A. dos
Dutra, Alberto Augusto Noronha
Clarêncio, Jorge
Oliveira, Camila Indiani de
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, Brasil
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil / Institute of Investigation in Immunology-III. Salvador, BA, Brasil
Abstract
Neutrophils are involved in the initial steps of most responses to pathogens. In the present study, we evaluated the effects of the interaction of apoptotic vs. necrotic human neutrophils on macrophage infection by Leishmania amazonensis. Phagocytosis of apoptotic, but not viable, neutrophils by Leishmania-infected macrophages led to an increase in parasite burden via a mechanism dependent on TGF-beta1 and PGE2. Conversely, infected macrophages' uptake of necrotic neutrophils induced killing of L. amazonensis. Leishmanicidal activity was dependent on TNF-alpha and neutrophilic elastase. Nitric oxide was not involved in the killing of parasites, but the interaction of necrotic neutrophils with infected macrophages resulted in high superoxide production, a process reversed by catalase, an inhibitor of reactive oxygen intermediate production. Initial events after Leishmania infection involve interactions with neutrophils; we demonstrate that phagocytosis of these cells in an apoptotic or necrotic stage can influence the outcome of infection, driving either parasite survival or destruction.
Keywords
Apoptosis
Necrosis
Phagocytosis
TGF- B1
NE
 
DeCS
Leishmaniose Cutânea/fisiopatologia
Macrófagos/parasitologia
Neutrófilos/imunologia
Neutrófilos/fisiologia
Animais
Apoptose
Catalase/farmacologia
Efeitos Psicossociais da Doença
Dinoprostona/fisiologia
Humanos
Leishmania mexicana/patogenicidade
Leishmania mexicana/fisiologia
Leishmaniose Cutânea/patologia
Necrose
Neutrófilos/patologia
Fagocitose
Superóxidos/metabolismo
Fator de Crescimento Transformador beta1/fisiologia
 
Publisher
Society for Leukocyte Biology
Citation
AFONSO, L. Interactions with apoptotic but not with necrotic neutrophils increase parasite burden in human macrophages infected with Leishmania amazonensis. Journal of Leukocyte Biology, v.84, n. 2, p.389-392, 2008.
DOI
10.1189/jlb.0108018
ISSN
0741-5400