| Author | Iborra, Salvador | |
| Author | Parody, Nuria | |
| Author | Abánades, Daniel Ruiz | |
| Author | Bonay, Pedro | |
| Author | Prates, Deboraci Brito | |
| Author | Novais, Fernanda Oliveira | |
| Author | Barral Netto, Manoel | |
| Author | Alonso, Carlos | |
| Author | Soto, Manuel | |
| Access date | 2014-03-13T19:19:59Z | |
| Available date | 2014-03-13T19:19:59Z | |
| Document date | 2008 | |
| Citation | IBORRA, S. et al. Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice. Microbes and Infection, v. 10, n. 10/11, p. 1133-1141, 2008. | pt_BR |
| ISSN | 1286-4579 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7408 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Masson SAS | pt_BR |
| Rights | open access | pt_BR |
| Title | Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.micinf.2008.06.002 | |
| Abstract | In the present work we analyze the antigenicity of Leishmania major ribosomal proteins (LRP) in infected BALB/c mice.We show that BALB/c
mice vaccinated with LRP in the presence of CpG oligodeoxynucleotides (CpG-ODN) were protected against the development of dermal pathology
and showed a reduction in the parasite load after challenge with L. major. This protection was associated with the induction of an IL-12 dependent
specific-IFN-g response mediated mainly by CD4þ T cell, albeit a minor contribution of CD8þ T cells cannot be ruled out. Induction of Th1
responses against LRP also resulted in a reversion of the Th2 responses associated with susceptibility. A marked reduction of IgG1 antibody titer
against parasite antigens besides an impaired IL-4 and IL-10 cytokine production by parasite specific T cells was observed. In addition, we show
that the administration of the LRP plus CpG-ODN preparation also conferred protection in the naturally resistant C57BL/6 mice. In this strain
protection was associated with a LRP specific IFN-g production in lymph nodes draining the challenge site. We believe that these evolutionary
conserved proteins, combined with adjuvants that favor Th1 responses, may be relevant components of a pan-Leishmania vaccine.
2008 Elsevier Masson SAS. All rights reserved | pt_BR |
| Affilliation | Instituto de Salud Carlos III. Centro Nacional de Microbiología. Unidad de Inmunología Viral. Majadahonda, Madrid, Spain | pt_BR |
| Affilliation | Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain | pt_BR |
| Affilliation | Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain | pt_BR |
| Affilliation | Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain | pt_BR |
| Affilliation | Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain | pt_BR |
| Subject | Leishmania | pt_BR |
| Subject | BALB/C mice | pt_BR |
| Subject | C57BL/6 mice | pt_BR |
| Subject | Th1/Th2 immune responses; | pt_BR |
| Subject | Ribosomal proteins | pt_BR |
| Subject | Vaccines | pt_BR |
| DeCS | Leishmania major/imunologia | pt_BR |
| DeCS | Vacinas contra Leishmaniose | pt_BR |
| DeCS | Leishmaniose Cutânea/prevenção & controle | pt_BR |
| DeCS | Oligodesoxirribonucleotídeos/imunologia | pt_BR |
| DeCS | Proteínas Ribossômicas/imunologia | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Feminino | pt_BR |
| DeCS | Imunoglobulina G/metabolismo | pt_BR |
| DeCS | Interferon gama/metabolismo | pt_BR |
| DeCS | Interleucina-10/metabolismo | pt_BR |
| DeCS | Interleucina-4/metabolismo | pt_BR |
| DeCS | Leishmaniose Cutânea/imunologia | pt_BR |
| DeCS | Camundongos | pt_BR |
| DeCS | Camundongos Endogâmicos BALB C | pt_BR |
| DeCS | Camundongos Endogâmicos C57BL | pt_BR |
| DeCS | Oligodesoxirribonucleotídeos/genética | pt_BR |
| DeCS | Oligodesoxirribonucleotídeos/metabolismo | pt_BR |
| DeCS | Proteínas de Protozoários/genética | pt_BR |
| DeCS | Proteínas de Protozoários/imunologia | pt_BR |
| DeCS | Proteínas de Protozoários/metabolismo | pt_BR |
| DeCS | Proteínas Ribossômicas/genética | pt_BR |
| DeCS | Proteínas Ribossômicas/metabolismo | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
