Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7408
Title: Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice
Authors: Iborra, Salvador
Parody, Nuria
Abánades, Daniel Ruiz
Bonay, Pedro
Prates, Deboraci Brito
Novais, Fernanda Oliveira
Barral Netto, Manoel
Alonso, Carlos
Soto, Manuel
Affilliation: Instituto de Salud Carlos III. Centro Nacional de Microbiología. Unidad de Inmunología Viral. Majadahonda, Madrid, Spain
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain
Universidad Autónoma de Madrid. Facultad de Ciencias. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain
Abstract: In the present work we analyze the antigenicity of Leishmania major ribosomal proteins (LRP) in infected BALB/c mice.We show that BALB/c mice vaccinated with LRP in the presence of CpG oligodeoxynucleotides (CpG-ODN) were protected against the development of dermal pathology and showed a reduction in the parasite load after challenge with L. major. This protection was associated with the induction of an IL-12 dependent specific-IFN-g response mediated mainly by CD4þ T cell, albeit a minor contribution of CD8þ T cells cannot be ruled out. Induction of Th1 responses against LRP also resulted in a reversion of the Th2 responses associated with susceptibility. A marked reduction of IgG1 antibody titer against parasite antigens besides an impaired IL-4 and IL-10 cytokine production by parasite specific T cells was observed. In addition, we show that the administration of the LRP plus CpG-ODN preparation also conferred protection in the naturally resistant C57BL/6 mice. In this strain protection was associated with a LRP specific IFN-g production in lymph nodes draining the challenge site. We believe that these evolutionary conserved proteins, combined with adjuvants that favor Th1 responses, may be relevant components of a pan-Leishmania vaccine. 2008 Elsevier Masson SAS. All rights reserved
Keywords: Leishmania
BALB/C mice
C57BL/6 mice
Th1/Th2 immune responses;
Ribosomal proteins
Vaccines
DeCS: Leishmania major/imunologia
Vacinas contra Leishmaniose
Leishmaniose Cutânea/prevenção & controle
Oligodesoxirribonucleotídeos/imunologia
Proteínas Ribossômicas/imunologia
Animais
Feminino
Imunoglobulina G/metabolismo
Interferon gama/metabolismo
Interleucina-10/metabolismo
Interleucina-4/metabolismo
Leishmaniose Cutânea/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Endogâmicos C57BL
Oligodesoxirribonucleotídeos/genética
Oligodesoxirribonucleotídeos/metabolismo
Proteínas de Protozoários/genética
Proteínas de Protozoários/imunologia
Proteínas de Protozoários/metabolismo
Proteínas Ribossômicas/genética
Proteínas Ribossômicas/metabolismo
Issue Date: 2008
Publisher: Elsevier Masson SAS
Citation: IBORRA, S. et al. Vaccination with the Leishmania major ribosomal proteins plus CpG oligodeoxynucleotides induces protection against experimental cutaneous leishmaniasis in mice. Microbes and Infection, v. 10, n. 10/11, p. 1133-1141, 2008.
DOI: 10.1016/j.micinf.2008.06.002
ISSN: 1286-4579
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Borra S Vaccination with....pdf565.15 kBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.