Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7412
Title: Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
Authors: Vinhas, Vera
Andrade, Bruno de Bezerril
Paes, Fábio
Bomura, Andréa
Clarêncio, Jorge
Miranda, José Carlos
Bafica, Andre Luiz Barbosa
Barral, Aldina Maria Prado
Barral Netto, Manoel
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
National Institutes of Health NIAID. National Institutes of Health. Immunobiology Section. Bethesda, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. / Salvador, BA, Brasil / Instituto de Investiga ção em Imunologia. Instituto do Milênio. SAlvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. / Salvador, BA, Brasil / Instituto de Investiga ção em Imunologia. Instituto do Milênio. SAlvador, BA, Brasil
Abstract: Experiments in animals verified that phlebotomine saliva enhances Leishmania infection, and vaccination with saliva prevents disease. We have shown that individuals from an endemic area of visceral leishmaniasis displayed robust antibody responses to saliva from the vector Lutzomyia longipalpis, which correlated with anti-parasite cellmediated immunity. Here, we explored human anti-saliva responses following exposure to sand flies, using an in vivo bite model in which normal volunteers were exposed four times to 30 laboratory-reared Lu. longipalpis. Following the third exposure, normal volunteers developed diverse dermatological reactions at the site of insect bite. Serum from normal volunteers displayed high levels of anti-salivary gland sonicate IgG1, IgG4 and IgE as well as several salivary gland proteins. Furthermore, following in vitro stimulation with salivary gland sonicate, there was an increased frequency of CD4+CD25+ and CD8+CD25+ T cells as well as IFN-c and IL-10 synthesis. Strikingly, 1 year after the first exposure, PBMC from the volunteers displayed recall IFN-c responses that correlated with a significant reduction in infection rates using a macrophage-lymphocyte autologous culture. Together, these data suggest that human immunization against sand fly saliva is feasible and recall responses are obtained even 1 year after exposure, opening perspectives for vaccination in man.
DeCS: Proteínas de Insetos/imunologia
Insetos Vetores/imunologia
Leishmaniose Visceral/prevenção & controle
Psychodidae/imunologia
Saliva/imunologia
Adulto
Animais
Western Blotting
Citocinas/sangue
Citocinas/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Citometria de Fluxo
Humanos
Imunoglobulina E/sangue
Imunoglobulina G/sangue
Memória Imunológica
Leishmaniose Visceral/transmissão
Masculino
Proteínas e Peptídeos Salivares/imunologia
Linfócitos T/imunologia
Issue Date: 2007
Publisher: WILEY-VCH Verlag GmbH & Co.
Citation: VINHAS, V. et al. Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis. European Journal of Immunology, v. 37, p. 3111–3121, 2007.
ISSN: 0014-2980
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Vinhas V Human anti-saliva....pdf339.03 kBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.