Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7436
Title: Adhesion molecule expression patterns indicate activation and recruitment of CD4+ T cells from the lymph node to the peripheral blood of early cutaneous leishmaniasis patients
Authors: Costa, Ronaldo P
Gollob, Kenneth J
Machado, Paulo Roberto
Bacellar, Maria Olívia Amado Ramos
Almeida, Roque Pacheco de
Barral, Aldina Maria Prado
Barral Netto, Manoel
Carvalho Filho, Edgar Marcelino
Dutra, Walderez Ornelas
Affilliation: Universidade Federal de Minas Gerais. Laboratory of Cell–Cell Interactions. Department of Morphology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gearis. ICB. Laboratory of Lymphocyte Biology. Department of Biochemistry-Immunolog. Belo Horizonte, Belo Horizonte, MG, Brasil
Universidade Federal da Bahia. HUPES. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. HUPES. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. HUPES. Serviço de Imunologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Universidade Federal da Bahia. HUPES. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal de Minas Gerais. Laboratory of Cell–Cell Interactions. Department of Morphology. Biological Sciences Institute. Belo Horizonte, MG, Brasil
Abstract: Adhesion molecules play a crucial role in cell migration and recruitment. Expression of adhesion molecules that preferentially address cells to inflammatory sites is a critical event in the formation and maintenance of leishmaniasis lesions. In this work, we analyzed the expression of CD11a, CD11b and CD62L, adhesion molecules involved in cell activation and circulation, in CD4+ and CD8+ T cells from peripheral blood and lymph nodes of patients with early cutaneous leishmaniasis. The percentage of expression of CD62L, CD11a and CD11b in total lymphocytes was lower in lymph nodes as compared to peripheral blood. Moreover, differences in adhesion molecule expression between blood and lymph nodes were more striking in CD4+ than CD8+ T cells. Stimulation of PBMC from leishmaniasis patients with soluble Leishmania antigens (SLA) lead to the expansion of CD4+CD62Lhigh cells, CD4+CD11b+ cells and to an increase in the intensity of expression of CD11a in CD4+, but not CD8+ T cells. Our data suggest that early activation events that occur in the lymph nodes of patients recently infected with Leishmania lead to changes in T cell adhesion molecule expression, favoring migration to the periphery and increasing the likelihood of further recruitment to lesion sites.
Keywords: Leishmaniasis
Adhesion molecules
Lymph node
Lymphocytes
Peripheral blood
DeCS: Linfócitos T CD4-Positivos/imunologia
Moléculas de Adesão Celular/metabolismo
Leishmaniose Cutânea/sangue
Leishmaniose Cutânea/imunologia
Linfonodos/imunologia
Ativação Linfocitária
Animais
Antígenos CD/análise
Antígenos CD/imunologia
Antígenos de Protozoários/imunologia
Linfócitos T CD8-Positivos/imunologia
Humanos
Imunofenotipagem
Leishmania/imunologia
Linfonodos/citologia
Issue Date: 2003
Publisher: Elsevier B.V
Citation: COSTA, R. P. et al. Adhesion molecule expression patterns indicate activation and recruitment of CD4+ T cells from the lymph node to the peripheral blood of early cutaneous leishmaniasis patients. Immunology Letters, v. 90, p. 155–159, 2003.
ISSN: 0165-2478
10.1016/j.imlet.2003.09.001
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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