Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7441
Title: Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers.
Authors: Pompeu, Margarida Maria de Lima
Brodskyn, Claudia Ida
Teixeira, M. J
Clarêncio, Jorge
Van Weyenbergh, Johan Jozef Rosa Maria
Coelho, I. C
Cardoso, Silvia Andrade
Barral, Aldina Maria Prado
Barral Netto, Manoel
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Universidade Federal do Ceará. Núcleo de Medicina Tropical. Fortaleza, CE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal do Ceará. Núcleo de Medicina Tropical. Fortaleza, CE, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
Abstract: The initial encounter of Leishmania cells and cells from the immune system is fundamentally important in the outcome of infection and determines disease development or resistance. We evaluated the anti-Leishmania amazonensis response of naive volunteers by using an in vitro priming (IVP) system and comparing the responses following in vivo vaccination against the same parasite. In vitro stimulation allowed us to distinguish two groups of individuals, those who produced small amounts of gamma interferon (IFN-gamma) (n = 16) (low producers) and those who produced large amounts of this cytokine (n = 16) (high producers). IFN-gamma production was proportional to tumor necrosis factor alpha and interleukin 10 (IL-10) levels but did not correlate with IL-5 production. Volunteers who produced small amounts of IFN-gamma in vitro remained low producers 40 days after vaccination, whereas high producers exhibited increased IFN-gamma production. However, 6 months after vaccination, all individuals tested produced similarly high levels of IFN-gamma upon stimulation of their peripheral blood mononuclear cells with Leishmania promastigotes, indicating that low in vitro producers respond slowly in vivo to vaccination. In high IFN-gamma producers there was an increased frequency of activated CD8(+) T cells both in vitro and in vivo compared to the frequency in low producers, and such cells were positive for IFN-gamma as determined by intracellular staining. Such findings suggest that IVP responses can be used to predict the pace of postvaccination responses of test volunteers. Although all vaccinated individuals eventually have a potent anti-Leishmania cell-mediated immunity (CMI) response, a delay in mounting the CMI response may influence resistance against leishmaniasis.
DeCS: Interferon gama/biossíntese
Leishmaniose/imunologia
Vacinas Protozoárias/imunologia
Adolescente
Adulto
Linfócitos T CD8-Positivos/imunologia
Previsões
Humanos
Imunidade Inata
Ativação Linfocitária
Masculino
Receptores de Interleucina-2/isolamento & purificação
Subpopulações de Linfócitos T/imunologia
Vacinação
Issue Date: 2001
Publisher: American Society for Microbiology.
Citation: POMPEU, M. M. et al. Differences in gamma interferon production in vitro predict the pace of the in vivo response to Leishmania amazonensis in healthy volunteers. Infection and Immunity, v. 69, n. 12, p. 7453-7460, 2001.
DOI: 10.1128/IAI.69.12.7453–7460.2001
ISSN: 0019-9567
Copyright: open access
Appears in Collections:IGM - Artigos de Periódicos

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