| Author | Veras, Patrícia Sampaio Tavares | |
| Author | Brodskyn, Claudia Ida | |
| Author | Balestieri, Filomena Maria Perrella | |
| Author | Freitas, Luiz Antonio Rodrigues de | |
| Author | Ramos, A. P. S | |
| Author | Queiroz, A. R. P | |
| Author | Barral, Aldina Maria Prado | |
| Author | Beverley, Stephen M | |
| Author | Barral Netto, Manoel | |
| Access date | 2014-03-25T17:21:29Z | |
| Available date | 2014-03-25T17:21:29Z | |
| Document date | 1999 | |
| Citation | VERAS, P. S. R. et al. A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis. Memórias do Instituto Oswaldo Cruz, v. 94, n. 4, p. 491-496, 1999. | pt_BR |
| ISSN | 0074-0276 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7444 | |
| Language | eng | pt_BR |
| Publisher | Fiocruz | pt_BR |
| Rights | open access | pt_BR |
| Title | A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis. | pt_BR |
| Type | Article | pt_BR |
| Abstract | E10-5A3 is a dhfr-ts- Leishmania major double knockout auxotrophic shown previously to induce substantial protection against virulent L. major infection in both genetically susceptible and resistant mice. We investigated the capacity of dhfr-ts- to protect against heterologous infection by L. amazonensis. The degree of protection was evaluated by immunization of BALB/c or C57BL/6 mice with E10-5A3, followed by L. amazonensis challenge. Whether immunized by subcutaneous (SC) or intravenous (IV) inoculation, susceptible and resistant mice displayed a partial degree of protection against challenge with virulent L. amazonensis. SC-immunized BALB/c mice developed lesions 40 to 65% smaller than non immunized mice, while IV immunization led to protection ranging from 40 to 75% in four out of six experiments compared to non immunized animals. The resistant C57BL/6 mice displayed comparable degrees of protection, 57% by SC and 49% by IV immunization. Results are encouraging as it has been previously difficult to obtain protection by SC vaccination against Leishmania, the preferred route for human immunization. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. Departamento de Fisiologia e Patologia. João Pessoa, PB, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Washington University School of Medicine. Department of Molecular Microbiology. St. Louis, MO, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, Brasil | pt_BR |
| Subject | Auxotrophic Leishmania major | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| Subject | Immunization | pt_BR |
| Subject | Cross | pt_BR |
| Subject | Protection | pt_BR |
| DeCS | Antígenos de Protozoários/administração & dosagem | pt_BR |
| DeCS | Leishmania major/imunologia | pt_BR |
| DeCS | Leishmaniose Cutânea/prevenção & controle | pt_BR |
| DeCS | Complexos Multienzimáticos/imunologia | pt_BR |
| DeCS | Vacinas Protozoárias/imunologia | pt_BR |
| DeCS | Tetra-Hidrofolato Desidrogenase/imunologia | pt_BR |
| DeCS | Timidilato Sintase/imunologia | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Leishmaniose Cutânea/imunologia | pt_BR |
| DeCS | Camundongos | pt_BR |
| DeCS | Camundongos Endogâmicos BALB C | pt_BR |
| DeCS | Camundongos Knockout | pt_BR |
| DeCS | Camundongos Mutantes | pt_BR |
