Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7444
Title: A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis.
Authors: Veras, Patrícia Sampaio Tavares
Brodskyn, Claudia Ida
Balestieri, Filomena Maria Perrella
Freitas, Luiz Antonio Rodrigues de
Ramos, A. P. S
Queiroz, A. R. P
Barral, Aldina Maria Prado
Beverley, Stephen M
Barral Netto, Manoel
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Paraíba. Laboratório de Tecnologia Farmacêutica. Departamento de Fisiologia e Patologia. João Pessoa, PB, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Washington University School of Medicine. Department of Molecular Microbiology. St. Louis, MO, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, Brasil
Abstract: E10-5A3 is a dhfr-ts- Leishmania major double knockout auxotrophic shown previously to induce substantial protection against virulent L. major infection in both genetically susceptible and resistant mice. We investigated the capacity of dhfr-ts- to protect against heterologous infection by L. amazonensis. The degree of protection was evaluated by immunization of BALB/c or C57BL/6 mice with E10-5A3, followed by L. amazonensis challenge. Whether immunized by subcutaneous (SC) or intravenous (IV) inoculation, susceptible and resistant mice displayed a partial degree of protection against challenge with virulent L. amazonensis. SC-immunized BALB/c mice developed lesions 40 to 65% smaller than non immunized mice, while IV immunization led to protection ranging from 40 to 75% in four out of six experiments compared to non immunized animals. The resistant C57BL/6 mice displayed comparable degrees of protection, 57% by SC and 49% by IV immunization. Results are encouraging as it has been previously difficult to obtain protection by SC vaccination against Leishmania, the preferred route for human immunization.
Keywords: Auxotrophic Leishmania major
Leishmania amazonensis
Immunization
Cross
Protection
DeCS: Antígenos de Protozoários/administração & dosagem
Leishmania major/imunologia
Leishmaniose Cutânea/prevenção & controle
Complexos Multienzimáticos/imunologia
Vacinas Protozoárias/imunologia
Tetra-Hidrofolato Desidrogenase/imunologia
Timidilato Sintase/imunologia
Animais
Leishmaniose Cutânea/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Camundongos Knockout
Camundongos Mutantes
Issue Date: 1999
Publisher: Fiocruz
Citation: VERAS, P. S. R. et al. A dhfr-ts- Leishmania major knockout mutant cross-protects against Leishmania amazonensis. Memórias do Instituto Oswaldo Cruz, v. 94, n. 4, p. 491-496, 1999.
ISSN: 0074-0276
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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