Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7445
Title: Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
Authors: Arruda, Sérgio Marcos
Chalhoub, Marcelo
Cardoso, Silvia Andrade
Barral Netto, Manoel
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, Brasil
SESAB. Hospital Octávio Mangabeira - HOM. Salvador, BA, Brasil
SESAB. Hospital Octávio Mangabeira - HOM. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, Brasil
Abstract: Evaluating human immune response to defined Mycobacterium tuberculosis antigens in patients with different clinical forms of tuberculosis may help in elucidating pathogenesis and in vaccine development. In the present report we evaluated the lymphocyte proliferation, cytokine production and natural killer cell cytotoxicity as parameters to screen four mycobacterial recombinant antigens. Pleural fluid mononuclear cells (PFMC) and peripheral blood mononuclear cells (PBMC) from 13 HIV-negative patients with tuberculous pleurisy, living in a tropical region of Brazil were used in these assays. Crude M. tuberculosis antigen and recombinant 70-, 65- and 38-kDa mycobacterial antigens, induced greater proliferation in PFMC than in PBMC. IFN-gamma, TNF-alpha, IL-4 and IL-10 were evaluated in the PFMC supernatants stimulated by these antigens. Both crude and 70-kDa antigens induced higher levels of IFN-gamma, TNF-alpha and IL-10. There was a significant positive correlation between IFN-gamma and the proliferative response induced by crude M. tuberculosis antigen, and an inverse correlation was identified between IL-10 and cell proliferation. IL-4 was not detected in the supernatants of pleural fluid mononuclear cell cultures stimulated by either crude, or recombinant antigens. TNF-alpha was detected in variable amounts in supernatants of PFMC stimulated by all antigens tested. Natural killer cytotoxicity was induced by both crude and 70-kDa antigen. Our results demonstrate that cells present at the site of disease recognized three of the antigens screened, as shown by lymphocyte proliferation and production of regulatory and inflammatory cytokines, and the results obtained with PFMC were consistently higher than those obtained with homologous PBMC.
DeCS: Antígenos de Bactérias/imunologia
Citocinas/sangue
Citotoxicidade Imunológica/imunologia
Ativação Linfocitária/imunologia
Mycobacterium tuberculosis/imunologia
Tuberculose Pleural/imunologia
Anticorpos Monoclonais
Citotoxicidade Imunológica/fisiologia
Ensaio de Imunoadsorção Enzimática
Humanos
Imunidade Celular/imunologia
Interferon gama/sangue
Interleucina-10/sangue
Células Matadoras Naturais/imunologia
Derrame Pleural/imunologia
Proteínas Recombinantes/imunologia
Tuberculose Pleural/microbiologia
Fator de Necrose Tumoral alfa/imunologia
Issue Date: 1998
Publisher: Elsevier Science B.V
Citation: ARRUDA, S. et al. Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil. Acta Tropica, v. 71, p. 1-15, 1998.
ISSN: 0001-706X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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