Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7454
Title: Cytotoxicity in patients with different clinical forms of Chagas' disease
Authors: Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Bulhões, M. A.
Souto, T.
Machado, W. C.
Barral Netto, Manoel
Affilliation: Universidade Federal da Bahia. Hospital Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil
Universidade Federal da Bahia. Hospital Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Integrado de Microbiologia e Imunoregulação. Salvador, BA, Brasil
Abstract: There are few studies on cell-mediated cytotoxicity in human Chagas' disease. In the present study, we evaluated peripheral blood mononuclear cell (PBMC) cytotoxicity activity from chagasic patients with different clinical forms of disease. To verify the cytotoxic response, we performed cell lysis assays using 51Cr-labelled K562 cells as targets. Results are reported as lytic units (LU = number of cells required for 30% lysis) per million PBMC. Exposure of patients' PBMC to Trypanosoma cruzi antigen led to an increase in cytotoxic activity compared with unstimulated patient cells against K562. Asymptomatic cardiomyopathy patients had higher responses (37.8 +/- 5.0 LU/10(6) PBMC; mean +/- s.d.) than indeterminate (11.5 +/- 3.6 LU/10(6) and symptomatic cardiomyopathy (7.8 +/- 2.5 LU/10(6)). Normal control PBMC stimulated with T. cruzi antigen had 4.36 +/- 1.31 LU/10(6)) PBMC against K562. Addition of recombinant interferon-gamma (IFN-gamma) did not lead to significant increase in cytotoxicity in any group of patients. On the other hand, recombinant human IL-12 significantly increased cytotoxic responses from symptomatic cardiomyopathy patients and normal controls who presented low levels of cytotoxicity induced by T. cruzi antigen. The combined use of IL-12 and a neutralizing anti-IFN-gamma antibody did not change IL-12-induced cytotoxic responses, showing the direct role of this cytokine on natural killer (NK) cells. NK cells were the main cells responsible for the lysis of K562 target cells as evidenced by testing cell subsets following magnetic cell sorting. These data demonstrate that chagasic patients with different clinical forms of disease have PBMC which respond to T. cruzi antigen with a cytotoxic response, and this response is up-regulated by IL-12.
Keywords: Chagas' disease
Citotoxicity
Interferon-gamma
Il-12
DeCS: Doença de Chagas/imunologia
Citotoxicidade Imunológica
Adulto
Idoso
Animais
Testes Imunológicos de Citotoxicidade
Humanos
munofenotipagem
Interferon gama/fisiologia
Interleucina-12/fisiologia
Leucemia Eritroblástica Aguda/imunologia
Meia-Idade
Células Tumorais Cultivadas
Issue Date: 1996
Publisher: Blackwell Science
Citation: BRODSKYN, C. I. et al. ytotoxicity in patients with different clinical forms of Chagas' disease. Clinical and Experimental Immunology, v. 105, n. 3, p. 450-455, 1996.
ISSN: 0009-9104
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Brodskyn C Cytotoxicity in....pdf208.78 kBAdobe PDFThumbnail
View/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.