| Author | Rodríguez Lay, Licel de los Angeles | |
| Author | Villalba, Maria Caridad Montalvo | |
| Author | Corredora, Marité Bello | |
| Author | Frómeta, Susel Sariego | |
| Author | Hernández, Jeny Marante | |
| Author | Carrera, Santiago Dueñas | |
| Author | Wonga, Meilin Sánchez | |
| Author | Samada, Marcia | |
| Author | Núñez, Milay Bello | |
| Author | Alonso, Lidunka Valdes | |
| Author | Silva Filho, Hermes Pedreira da | |
| Author | Hübschenf, Judith M. | |
| Author | Reis, Mitermayer Galvão dos | |
| Access date | 2014-04-03T19:02:16Z | |
| Available date | 2014-04-03T19:02:16Z | |
| Document date | 2012 | |
| Citation | RODRIGUEZ LAY, L. de los A. et al. HCV genotype determination in monoinfected and HIV co-infected patients in Cuba. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 106, n. 12, p. 711–717, 2012. | pt_BR |
| ISSN | 1878-3503 | |
| ISSN | 10.1016/j.trstmh.2012.08.001 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7493 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Ltd | pt_BR |
| Rights | open access | pt_BR |
| Title | HCV genotype determination in monoinfected and HIV co-infected patients in Cuba. | pt_BR |
| Type | Article | pt_BR |
| Abstract | With the aim to characterize the HCV genotype distribution in Cuba, sera were collected
from two subgroups: HCV-monoinfected and HCV/HIV co-infected patients. A combination
of reverse transcription-PCR using genotype-specific primers, restriction fragment
length polymorphism and sequencing was used to determine the genotype of 84 samples.
Seventy-nine (94%) showed single infections (10 [12%] were genotype 1a and 69 [82%]
genotype 1b) and 5 (6%) samples corresponded to mixed infections (2 [2%] with genotypes
1a/3a and 1 sample [1%] each with 1b/3a, 1b/4a and 1a/1b/3a). HCV/HIV co-infected
subjects had a higher frequency of mixed infections (p=0.08), infection with genotype 3a
(p=0.18) and for the first time genotype 4a was found. There was no association of any demographic
characteristics with any specific genotype although HCV/HIV co-infected patients
showed a tendency to have mixed genotypes in those older than 45 years of age (p=0.11).
Phylogenetic analysis showed that HCV isolates clustered with subtypes 1b (n=15, maximal
genetic distance 2.51%) and 1a (n=2, maximal genetic distance 0.35%). This report presents
the prevalence of HCV genotypes in monoinfected and HIV co-infected patients, mixed HCV
infections in HCV/HIV co-infected men who have sex with men with high-risk sexual practices
and for the first time identifies that the uncommon genotype 4a can be present in a
patient co-infected with HIV. | pt_BR |
| Affilliation | Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba | pt_BR |
| Affilliation | Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba | pt_BR |
| Affilliation | Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba | pt_BR |
| Affilliation | Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba | pt_BR |
| Affilliation | Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba | pt_BR |
| Affilliation | Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba | pt_BR |
| Affilliation | Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba | pt_BR |
| Affilliation | Centro de Investigaciones Médico-Quirúrgicas. CIMEQ. Havana, Cuba | pt_BR |
| Affilliation | Ivan Portuondo General Hospital. San Antonio de los Baños. Havana, Cuba | pt_BR |
| Affilliation | Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
| Affilliation | Institute of Immunology. CRP-Santé/Laboratoire National de Santé. Luxembourg, Luxembourg | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil | pt_BR |
| Subject | HCV | pt_BR |
| Subject | HIV | pt_BR |
| Subject | HCV/HIV co-infection | pt_BR |
| Subject | Molecular epidemiology | pt_BR |
| Subject | Phylogenetic analysis | pt_BR |
| Subject | Risk groups | pt_BR |
| Subject | RNA Viral/genética | pt_BR |
| DeCS | Coinfecção/virologia | pt_BR |
| DeCS | Infecções por HIV/virologia | pt_BR |
| DeCS | Hepacivirus/genética | pt_BR |
| DeCS | Hepatite C/virologia | pt_BR |
| DeCS | Adolescente | pt_BR |
| DeCS | Adulto | pt_BR |
| DeCS | Idoso | pt_BR |
| DeCS | Criança | pt_BR |
| DeCS | Coinfecção/epidemiologia | pt_BR |
| DeCS | Cuba/epidemiologia | pt_BR |
| DeCS | Primers do DNA/genética | pt_BR |
| DeCS | Feminino | pt_BR |
| DeCS | Genótipo | pt_BR |
| DeCS | Infecções por HIV/epidemiologia | pt_BR |
| DeCS | Hepacivirus/classificação | pt_BR |
| DeCS | Hepatite C/diagnóstico | pt_BR |
| DeCS | Hepatite C/epidemiologia | pt_BR |
| DeCS | Homossexualidade Masculina | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Meia-Idade | pt_BR |
| DeCS | Epidemiologia Molecular | pt_BR |
