Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7493
Title: HCV genotype determination in monoinfected and HIV co-infected patients in Cuba.
Authors: Rodríguez Lay, Licel de los Angeles
Villalba, Maria Caridad Montalvo
Corredora, Marité Bello
Frómeta, Susel Sariego
Hernández, Jeny Marante
Carrera, Santiago Dueñas
Wonga, Meilin Sánchez
Samada, Marcia
Núñez, Milay Bello
Alonso, Lidunka Valdes
Silva Filho, Hermes Pedreira da
Hübschenf, Judith M.
Reis, Mitermayer Galvão dos
Affilliation: Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Center for Genetic Engineering and Biotechnology (CIGB). Havana, Cuba
Centro de Investigaciones Médico-Quirúrgicas. CIMEQ. Havana, Cuba
Ivan Portuondo General Hospital. San Antonio de los Baños. Havana, Cuba
Institute for Tropical Medicine “Pedro Kourí”. Department of Virology. National Reference Laboratory on Viral Hepatitis. Havana City, Cuba
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Institute of Immunology. CRP-Santé/Laboratoire National de Santé. Luxembourg, Luxembourg
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil
Abstract: With the aim to characterize the HCV genotype distribution in Cuba, sera were collected from two subgroups: HCV-monoinfected and HCV/HIV co-infected patients. A combination of reverse transcription-PCR using genotype-specific primers, restriction fragment length polymorphism and sequencing was used to determine the genotype of 84 samples. Seventy-nine (94%) showed single infections (10 [12%] were genotype 1a and 69 [82%] genotype 1b) and 5 (6%) samples corresponded to mixed infections (2 [2%] with genotypes 1a/3a and 1 sample [1%] each with 1b/3a, 1b/4a and 1a/1b/3a). HCV/HIV co-infected subjects had a higher frequency of mixed infections (p=0.08), infection with genotype 3a (p=0.18) and for the first time genotype 4a was found. There was no association of any demographic characteristics with any specific genotype although HCV/HIV co-infected patients showed a tendency to have mixed genotypes in those older than 45 years of age (p=0.11). Phylogenetic analysis showed that HCV isolates clustered with subtypes 1b (n=15, maximal genetic distance 2.51%) and 1a (n=2, maximal genetic distance 0.35%). This report presents the prevalence of HCV genotypes in monoinfected and HIV co-infected patients, mixed HCV infections in HCV/HIV co-infected men who have sex with men with high-risk sexual practices and for the first time identifies that the uncommon genotype 4a can be present in a patient co-infected with HIV.
Keywords: HCV
HIV
HCV/HIV co-infection
Molecular epidemiology
Phylogenetic analysis
Risk groups
RNA Viral/genética
DeCS: Coinfecção/virologia
Infecções por HIV/virologia
Hepacivirus/genética
Hepatite C/virologia
Adolescente
Adulto
Idoso
Criança
Coinfecção/epidemiologia
Cuba/epidemiologia
Primers do DNA/genética
Feminino
Genótipo
Infecções por HIV/epidemiologia
Hepacivirus/classificação
Hepatite C/diagnóstico
Hepatite C/epidemiologia
Homossexualidade Masculina
Humanos
Masculino
Meia-Idade
Epidemiologia Molecular
Issue Date: 2012
Publisher: Elsevier Ltd
Citation: RODRIGUEZ LAY, L. de los A. et al. HCV genotype determination in monoinfected and HIV co-infected patients in Cuba. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 106, n. 12, p. 711–717, 2012.
ISSN: 1878-3503
10.1016/j.trstmh.2012.08.001
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Rodriguez Lay LLA HCV genotype determination....pdf262.97 kBAdobe PDFThumbnail
View/Open


FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.