| Autor | Cerqueira, Gustavo Maia de | |
| Autor | McBride, Alan John Alexander | |
| Autor | Queiroz, Adriano | |
| Autor | Pinto, Luciano S | |
| Autor | Silva, Éverton Fagonde | |
| Autor | Hartskeerl, Rudy A | |
| Autor | Reis, Mitermayer Galvão dos | |
| Autor | Ko, Albert Icksang | |
| Autor | Dellagostin, Odir Antonio | |
| Data de acesso | 2014-04-07T18:52:34Z | |
| Data de disponibilização | 2014-04-07T18:52:34Z | |
| Data do publicação | 2010 | |
| Citação | CERQUEIRA, G. M. et al. Monitoring Leptospira strain collections: the need for quality control. American Journal of Tropical Medicine and Hygiene, v. 82, n. 1, p. 83-87, 2010. | pt_BR |
| ISSN | 1476-1645 | |
| URI | https://www.arca.fiocruz.br/handle/icict/7502 | |
| Idioma | eng | pt_BR |
| Editor | American Society of Tropical Medicine and Hygiene | pt_BR |
| Direito Autoral | open access | pt_BR |
| Título | Monitoring Leptospira strain collections: the need for quality control. | pt_BR |
| Tipo do documento | Article | pt_BR |
| DOI | 10.4269/ajtmh.2010.09-0558 | |
| Resumo em Inglês | The purpose of this study was to perform a 16S sequence-based quality control of two Leptospira strain collections. 16S rRNA gene sequencing was used to verify two Leptospira reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify Leptospira beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of Leptospira reference collections | pt_BR |
| Afiliação | Federal University of Pelotas. Biotechnology Centre, Pelotas, RS, Brasil | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Afiliação | Universidade Federal da Bahia. Hospital das Clínicas. Salvador, BA, Brasil | pt_BR |
| Afiliação | Federal University of Pelotas. Biotechnology Centre, Pelotas, RS, Brasil | pt_BR |
| Afiliação | Federal University of Pelotas. Biotechnology Centre, Pelotas, RS, Brasil | pt_BR |
| Afiliação | Department of Biomedical Research. Royal Tropical Institute. Amsterdam, The Netherlands | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Afiliação | Division of Infectious Diseases Weill Medical College of Cornell University. New York, NY | pt_BR |
| Afiliação | Federal University of Pelotas. Biotechnology Centre, Pelotas, RS, Brasil | pt_BR |
| DeCS | Leptospira/isolamento & purificação | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Sequência de Bases | pt_BR |
| DeCS | Primers do DNA | pt_BR |
| DeCS | Leptospira/genética | pt_BR |
| DeCS | RNA Ribossômico 16S/genética | pt_BR |
| DeCS | Especificidade da Espécie | pt_BR |
