Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7520
Title: Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates.
Authors: Cerqueira, Gustavo Maia de
McBride, Alan John Alexander
Picardeau, Mathieu
Ribeiro, Samuel G
Moreira, Ângela N
Morel, Viviane
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Dellagostin, Odir Antonio
Affilliation: Federal University of Pelotas. Biotechnology Centre. Pelotas, RS, Brasil / Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France
Federal University of Pelotas. Biotechnology Centre. Pelotas, RS, Brasil
Federal University of Pelotas. Biotechnology Centre. Pelotas, RS, Brasil
Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, NY, USA
Federal University of Pelotas. Biotechnology Centre. Pelotas, RS, Brasil
Abstract: The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, the ligB gene was distributed ubiquitously among all pathogenic strains. None of the lig genes were detected among intermediate or saprophytic Leptospira species. It was also shown that, similar to the previously characterized secY gene, a short specific PCR fragment of ligB could be used to correctly identify pathogenic Leptospira species. These findings demonstrate that ligB is widely present among pathogenic strains and may be useful for their reliable identification and classification.
DeCS: Antígenos de Bactérias/genética
Leptospira/classificação
Leptospira/genética
Antígenos de Bactérias/química
Antígenos de Bactérias/imunologia
Técnicas de Tipagem Bacteriana
Sequência de Bases
DNA Bacteriano/genética
Regulação Bacteriana da Expressão Gênica/fisiologia
Leptospira/imunologia
Leptospira/patogenicidade
Dados de Sequência Molecular
Filogenia
RNA Bacteriano/genética
Issue Date: 2009
Publisher: SGM
Citation: CERQUEIRA, G. M. et al. Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates. Journal of Medical Microbiology, v. 58, n. 9, p. 1173–1181, 2009.
DOI: 10.1099/jmm.0.009175-0
ISSN: 0022-2615
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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