Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7521
Title: Analysis of Schistosoma mansoni population structure using total fecal egg sampling
Authors: Blank, Walter A.
Reis, Eliana Almeida Gomes
Thiong'o, Fred W.
Braghiroli, João F.
Santos, Jarbas M.
Melo, Paulo Roberto Santana de
Guimarães, Isabel C. S.
Silva, Luciano Kalabric
Carmo, Theomira Mauadie de Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
University of Nairobi. Nairobi, Kenya / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Federal University of Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Center for Global Health and Diseases. Case Western Reserve University. Cleveland, Ohio.
Abstract: Many parasite populations are difficult to sample because they are not uniformly distributed between several host species and are often not easily collected from the living host, thereby limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of eggs, in aggregate, from the stools of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults. Here, we show that genotyping of parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. Microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Preferential amplification of smaller alleles, and stutter PCR products, had negligible effect on measurement of genetic differentiation. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per infected individual and by reducing bias.
Keywords: Schistosoma Mansoni
Fecal egg sampling
DeCS: DNA de Helmintos/química
Fezes/parasitologia
Repetições de Microssatélites/genética
Schistosoma mansoni/isolamento & purificação
Esquistossomose mansoni/parasitologia
Eletroforese em Gel de Poliacrilamida
Animais
Brasil
Feminino
Frequência do Gene
Genótipo
Humanos
Quênia
Masculino
Óvulo
Reação em Cadeia da Polimerase
Schistosoma mansoni/classificação
Schistosoma mansoni/genética
Análise de Sequência
Issue Date: 2009
Publisher: National Institute of Health
Citation: BLANK, W. A. et al. Analysis of Schistosoma mansoni population structure using total fecal egg sampling. Journal of Parasitolofy, v. 95, n. 4, p. 881-889, 2009.
DOI: 10.1645/GE-1895.1
ISSN: 1937-2345
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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