Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7523
Title: Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
Authors: Athanazio, Daniel Abensur
Silva, Éverton Fagonde
Santos, Cleiton Silva
Rocha, Gustavo M
Santos, Marcos André Vannier dos
McBride, Alan John Alexander
Ko, Albert Icksang
Reis, Mitermayer Galvão dos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Pelotas. Biotechnology Center. Rio Grande do Sul, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. NY, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brasil
Abstract: Leptospirosis continues to be a disease with a poorly understood pathogenesis. The experimental rat model is amenable for the investigation of leptospiral dissemination, tropism, persistence of renal colonization and factors related to disease resistance. In this study, Wistar rats were infected intraperitoneally with virulent Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130. The detection of leptospires in tissue samples was based on culture, silver staining and immunofluorescence techniques. An inoculum of 10,000 leptospires induced colonization in 50% of rats and colonization persisted for the 4-month period of the study. Dissemination kinetics revealed that renal colonization took place 7-9 days after infection, with no underlying histopathology. The peak leptospiral load occurred on day 5 post-infection, followed by rapid clearance in all tissues except the kidneys, where dense leptospiral aggregates persisted in the renal tubules. We conclude that the experimental rat model is suitable for studies contributing towards the understanding of the mechanisms of colonization and resistance to severe disease in leptospirosis
Keywords: Leptospirosis
Leptospira
Rats
Animal disease models
DeCS: Modelos Animais de Doenças
Rim/patologia
Leptospira interrogans/patogenicidade
Leptospirose/patologia
Animais
Feminino
Rim/microbiologia
Leptospirose/microbiologia
Microscopia Eletrônica de Varredura
Ratos
Ratos Wistar
Virulência
Issue Date: 2008
Publisher: Elsevier B.V
Citation: ATHANAZIO, D. A. et al. Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans. Acta Tropica, v. 105, n. 2, p. 176-180, 2008.
ISSN: 0001-706X
10.1016/j.actatropica.2007.10.012
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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