| Author | Silva, Éverton Fagonde | |
| Author | Santos, Cleiton Silva | |
| Author | Athanazio, Daniel Abensur | |
| Author | Seyffert, Núbia | |
| Author | Seixas, Fabiana K | |
| Author | Cerqueira, Gustavo Maia de | |
| Author | Fagundes, Michel Q | |
| Author | Brod, Claudiomar Soares | |
| Author | Reis, Mitermayer Galvão dos | |
| Author | Dellagostin, Odir Antonio | |
| Author | Ko, Albert Icksang | |
| Access date | 2014-04-24T13:23:13Z | |
| Available date | 2014-04-24T13:23:13Z | |
| Document date | 2008 | |
| Citation | SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008. | pt_BR |
| ISSN | 0264-410X | |
| URI | https://www.arca.fiocruz.br/handle/icict/7532 | |
| Language | eng | pt_BR |
| Publisher | National Institute of Health | pt_BR |
| Rights | open access | pt_BR |
| Title | Characterization of virulence of Leptospira isolates in a hamster model. | pt_BR |
| Type | Article | pt_BR |
| Abstract | Effort has been made to identify protective antigens in order to develop a recombinant vaccine against leptospirosis. Several attempts failed to conclusively demonstrate efficacy of vaccine candidates due to the lack of an appropriate model of lethal leptospirosis. The purposes of our study were: (i) to test the virulence of leptospiral isolates from Brazil, which are representative of important serogroups that cause disease in humans and animals; and (ii) to standardize the lethal dose 50% (LD(50)) for each of the virulent strains using a hamster (Mesocricetus auratus) model. Five of seven Brazilian isolates induced lethality in a hamster model, with inocula lower than 200 leptospires. Histopathological examination of infected animals showed typical lesions found in both natural and experimental leptospirosis. Results described here demonstrated the potential use of Brazilian isolates as highly virulent strains in challenge experiments using hamster as an appropriate animal model for leptospirosis. Furthermore these strains may be useful in heterologous challenge studies which aim to evaluate cross-protective responses induced by sub-unit vaccine candidates. | pt_BR |
| Affilliation | Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil | pt_BR |
| Affilliation | Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil | pt_BR |
| Affilliation | Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil | pt_BR |
| Affilliation | Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil | pt_BR |
| Affilliation | Faculdade de Medicina Veterinária. Centro de Controle de Zoonoses. Pelotas, RS, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, USA | pt_BR |
| Subject | Leptospira | pt_BR |
| Subject | Leptospirosis | pt_BR |
| Subject | lethal dose | pt_BR |
| Subject | isolation; animal model; virulence | pt_BR |
| Subject | Animal model | pt_BR |
| Subject | Virulence | pt_BR |
| DeCS | Modelos Animais de Doenças | pt_BR |
| DeCS | Leptospira/patogenicidade | pt_BR |
| DeCS | Leptospirose/microbiologia | pt_BR |
| DeCS | Animais | pt_BR |
| DeCS | Cricetinae | pt_BR |
| DeCS | Feminino | pt_BR |
| DeCS | Humanos | pt_BR |
| DeCS | Rim/patologia | pt_BR |
| DeCS | Dose Letal Mediana | pt_BR |
| DeCS | Pulmão/patologia | pt_BR |
| DeCS | Masculino | pt_BR |
| DeCS | Mesocricetus | pt_BR |
| DeCS | Análise de Sobrevida | pt_BR |
| DeCS | Virulência | pt_BR |
