Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7532
Title: Characterization of virulence of Leptospira isolates in a hamster model.
Authors: Silva, Éverton Fagonde
Santos, Cleiton Silva
Athanazio, Daniel Abensur
Seyffert, Núbia
Seixas, Fabiana K
Cerqueira, Gustavo Maia de
Fagundes, Michel Q
Brod, Claudiomar Soares
Reis, Mitermayer Galvão dos
Dellagostin, Odir Antonio
Ko, Albert Icksang
Affilliation: Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
Faculdade de Medicina Veterinária. Centro de Controle de Zoonoses. Pelotas, RS, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, USA
Abstract: Effort has been made to identify protective antigens in order to develop a recombinant vaccine against leptospirosis. Several attempts failed to conclusively demonstrate efficacy of vaccine candidates due to the lack of an appropriate model of lethal leptospirosis. The purposes of our study were: (i) to test the virulence of leptospiral isolates from Brazil, which are representative of important serogroups that cause disease in humans and animals; and (ii) to standardize the lethal dose 50% (LD(50)) for each of the virulent strains using a hamster (Mesocricetus auratus) model. Five of seven Brazilian isolates induced lethality in a hamster model, with inocula lower than 200 leptospires. Histopathological examination of infected animals showed typical lesions found in both natural and experimental leptospirosis. Results described here demonstrated the potential use of Brazilian isolates as highly virulent strains in challenge experiments using hamster as an appropriate animal model for leptospirosis. Furthermore these strains may be useful in heterologous challenge studies which aim to evaluate cross-protective responses induced by sub-unit vaccine candidates.
Keywords: Leptospira
Leptospirosis
lethal dose
isolation; animal model; virulence
Animal model
Virulence
DeCS: Modelos Animais de Doenças
Leptospira/patogenicidade
Leptospirose/microbiologia
Animais
Cricetinae
Feminino
Humanos
Rim/patologia
Dose Letal Mediana
Pulmão/patologia
Masculino
Mesocricetus
Análise de Sobrevida
Virulência
Issue Date: 2008
Publisher: National Institute of Health
Citation: SILVA, E. F. et al. Characterization of virulence of Leptospira isolates in a hamster model. Vaccine, v. 26, n. 31, p. 3892-3896, 2008.
ISSN: 0264-410X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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