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GENETIC DIVERSITY OF THE LEPTOSPIRAL IMMUNOGLOBULIN-LIKE (LIG) GENES IN PATHOGENIC LEPTOSPIRA SPP.
DNA Bacteriano/genética
Variação Genética
Animais
Leptospira/fisiologia
Leptospira/genética
Leptospira/imunologia
Leptospirose/imunologia
Dados de Sequência Molecular
Filogenia
Análise de Sequência de DNA
Análise de Sequência de Proteína
Author
Affilliation
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
David Geffen School of Medicine at UCLA. Department of Biomathematics. Department of Human Genetics. Department of Biostatistics. Los Angeles, CA, USA
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
National Animal Disease Centre. Agricultural Research Service. U.S. Department of Agriculture Ames. Iowa, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, NY, USA
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
David Geffen School of Medicine at UCLA. Department of Biomathematics. Department of Human Genetics. Department of Biostatistics. Los Angeles, CA, USA
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
National Animal Disease Centre. Agricultural Research Service. U.S. Department of Agriculture Ames. Iowa, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / David Geffen School of Medicine at UCLA. Department of Medicine. Los Angeles, CA, USA
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, NY, USA
Universidade Federal de Pelotas. Centro de Biotecnologia. Pelotas, RS, Brasil
Abstract
Recent serologic, immunoprotection, and pathogenesis studies identified the Lig proteins as key
virulence determinants in interactions of leptospiral pathogens with the mammalian host. We
examined the sequence variation and recombination patterns of ligA, ligB, and ligC among 10
pathogenic strains from five Leptospira species. All strains were found to have intact ligB genes and
genetic drift accounting for most of the ligB genetic diversity observed. The ligA gene was found
exclusively in L. interrogans and L. kirschneri strains, and was created from ligB by a two-step partial
gene duplication process. The aminoterminal domain of LigB and the LigA paralog were essentially
identical (98.5 ± 0.8% mean identity) in strains with both genes. Like ligB, ligC gene variation also
followed phylogenetic patterns, suggesting an early gene duplication event. However, ligC is a
pseudogene in several strains, suggesting that LigC is not essential for virulence. Two ligB genes
and one ligC gene had mosaic compositions and evidence for recombination events between related
Leptospira species was also found for some ligA genes. In conclusion, the results presented here
indicate that Lig diversity has important ramifications for the selection of Lig polypeptides for use
in diagnosis and as vaccine candidates. This sequence information will aid the identification of highly
conserved regions within the Lig proteins and improve upon the performance characteristics of the
Lig proteins in diagnostic assays and in subunit vaccine formulations with the potential to confer
heterologous protection.
DeCS
Antígenos de Bactérias/genéticaDNA Bacteriano/genética
Variação Genética
Animais
Leptospira/fisiologia
Leptospira/genética
Leptospira/imunologia
Leptospirose/imunologia
Dados de Sequência Molecular
Filogenia
Análise de Sequência de DNA
Análise de Sequência de Proteína
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