Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7558
Title: Diagnostic performance of anti-beta2 glycoprotein I and anticardiolipin assays for clinical manifestations of the antiphospholipid syndrome.
Authors: Santiago, Mittermayer Barreto
Martinelli, Reinaldo
Reis, Mitermayer Galvão dos
Reis, Eliana Almeida Gomes
Ko, Albert Icksang
Fontes, Roberto Dias
Silva, Moacir Paranhos
Nascimento, Eliane Góes
Jesus, Rogério de
Pierangeli, Silvia
Espinola, Ricardo
Gharavi, Azzudin
Affilliation: Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Hospital Santa Izabel. Núcleo de Reumatologia do Serviço de Clínica Médica. Salvador, BA, Brasil
Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Weill Medical College. New York, NY, USA
COAS/DST/SESAB. Salvador, BA, Brasil
PIEJ/SESAB. Jequié, Brasil
PIEJ/SESAB. Jequié, Brasil
Morehouse School of Medicine. Atlanta, GA, USA
Morehouse School of Medicine. Atlanta, GA, USA
Morehouse School of Medicine. Atlanta, GA, USA
Abstract: The objective of the present study was to analyse the performance of the tests for detection of anti-beta2 glycoprotein I (beta2 GP I) and anticardiolipin (aCL) antibodies for identification of clinical manifestations of the antiphospholipid syndrome (APS). Patients with systemic lupus erythematosus (SLE) as well as carriers of infectious diseases such as Kala-azar, syphilis and leptospirosis were studied. Particular interest was given to the presence of clinical complications related to APS. Anticardiolipin and anti-beta2 GP I antibodies were searched using an enzyme-linked immunosorbent assay (ELISA) assay. Clinical manifestations of APS were observed in 34 of the 152 patients (22.3%) with SLE and no patient with infectious disease had such manifestations. Antibodies to cardiolipin in moderate or high levels and anti-beta2 GP I were detected in 55 of 152 (36.1%) and 36 of 152 (23.6%) patients with SLE, respectively, and in 2 of 30 (6.6%) and 16 of 30 (53.3%) patients with Kala-azar, in 9 of 39 (23%) and 6 of 34 (17.6%) patients with leptospirosis, and 14 of 74 (18.9%) and 8 of 70 (11.4%) cases of syphilis, respectively. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and likelihood ratio (LR) of the anti-beta2 GP I test for the identification of the clinical manifestation of APS were, respectively, 29% [95% confidence interval (CI) = 24%-34%], 78% (95% CI = 73-83%), 15% (95% CI = 11-19%), 89% (95% CI = 85-93%) and 1.38. Regarding the aCL assay, the figure was 29% (95% CI = 24-34%), 76% (95% CI = 71-81%), 14% (95% CI = 10-18%), 89% (95% CI = 86-92%) and 1.26. As the validity and performance of the anti-beta2 GP I assay were similar to the aCL in demonstrating the presence of clinical phenomena associated with APS and due to the difficulties in performing as well as the lack of standardisation of the anti-beta2 GP I test, we suggest that the test for aCL should continue to be the first one performed when the presence of APS is suspected
Keywords: Anticardiolipin
Antiphospholipid
Diagnostic test
Systemic lupus erythematosus
Anticorpos Anticardiolipina/imunologia
Síndrome Antifosfolipídica/sangue
DeCS: Anticorpos Anticardiolipina/sangue
Síndrome Antifosfolipídica/diagnóstico
Glicoproteínas/sangue
Adulto
Síndrome Antifosfolipídica/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Glicoproteínas/imunologia
Humanos
Infecção/sangue
Infecção/diagnóstico
Lúpus Eritematoso Sistêmico/sangue
Lúpus Eritematoso Sistêmico/diagnóstico
Masculino
Meia-Idade
beta 2-Glicoproteína I
Issue Date: 2004
Publisher: Clinical Rheumatology
Citation: SANTIAGO, M. B. et al. Diagnostic performance of anti-beta2 glycoprotein I and anticardiolipin assays for clinical manifestations of the antiphospholipid syndrome. Clinical Rheumatology, v. 23, n. 6, p. 485-489, 2004.
DOI: 10.1007/s10067-004-0924-5
ISSN: 0770-3198
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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