Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7561
Title: Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily
Authors: Matsunaga, James
Barocchi, Michele A
Croda, Julio Henrique Rosa
Young, Tracy A
Sanchez, Yolanda
Siqueira, Isadora Cristina de
Bolin, Carole A
Reis, Mitermayer Galvão dos
Riley, Lee Woodland
Haake, David A
Ko, Albert Icksang
Affilliation: Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / Department of Medicine. UCLA School of Medicine. Los Angeles, CA, USA.
Division of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley Berkeley, CA, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA.
Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / Department of Medicine. UCLA School of Medicine. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
College of Veterinary Medicine. Michigan State University. East Lansing, MI, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil.
Division of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley Berkeley, CA, USA.
Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, CA, USA / Department of Medicine. UCLA School of Medicine. Los Angeles, CA, USA.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Diseases. Weill Medical College of Cornell University. New York, NY, USA.
Abstract: Proteins with bacterial immunoglobulin-like (Big) domains, such as the Yersinia pseudotuberculosis invasin and Escherichia coli intimin, are surface-expressed proteins that mediate host mammalian cell invasion or attachment. Here, we report the identification and characterization of a new family of Big domain proteins, referred to as Lig (leptospiral Ig-like) proteins, in pathogenic Leptospira. Screening of L. interrogans and L. kirschneri expression libraries with sera from leptospirosis patients identified 13 lambda phage clones that encode tandem repeats of the 90 amino acid Big domain. Two lig genes, designated ligA and ligB, and one pseudogene, ligC, were identified. The ligA and ligB genes encode amino-terminal lipoprotein signal peptides followed by 10 or 11 Big domain repeats and, in the case of ligB, a unique carboxy-terminal non-repeat domain. The organization of ligC is similar to that of ligB but contains mutations that disrupt the reading frame. The lig sequences are present in pathogenic but not saprophytic Leptospira species. LigA and LigB are expressed by a variety of virulent leptospiral strains. Loss of Lig protein and RNA transcript expression is correlated with the observed loss of virulence during culture attenuation of pathogenic strains. High-pressure freeze substitution followed by immunocytochemical electron microscopy confirmed that the Lig proteins were localized to the bacterial surface. Immunoblot studies with patient sera found that the Lig proteins are a major antigen recognized during the acute host infection. These observations demonstrate that the Lig proteins are a newly identified surface protein of pathogenic Leptospira, which by analogy to other bacterial immunoglobulin superfamily virulence factors, may play a role in host cell attachment and invasion during leptospiral pathogenesis.
DeCS: Anticorpos Antibacterianos/genética
Proteínas de Bactérias/metabolismo
Leptospira/genética
Leptospira/metabolismo
Proteínas de Membrana/metabolismo
Sequência de Aminoácidos
Animais
Anticorpos Antibacterianos/química
Proteínas de Bactérias/química
Regulação Bacteriana da Expressão Gênica
Humanos
Imunoglobulinas/genética
Leptospira/imunologia
Proteínas de Membrana/química
Modelos Moleculares
Dados de Sequência Molecular
Estrutura Terciária de Proteína
Issue Date: 2003
Publisher: National Institute of Health
Citation: MATSUNAGA, J. et al. Pathogenic Leptospira species express surface-exposed proteins belonging to the bacterial immunoglobulin superfamily. Molecular Microbiology, v. 49, n. 4, p. 929-945, 2003.
ISSN: 0950-382X
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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