Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7566
Title: Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen
Authors: Barocchi, Michele A
Ko, Albert Icksang
Reis, Mitermayer Galvão dos
McDonald, Kent L
Riley, Lee Woodland
Affilliation: Division of Infectious Diseases and Immunity. School of Public Health. Berkeley, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Diseases. Weil Medical College of Cornell University. New York, NY
UC Berkeley Electron Microscopy Laboratory. University of California. Berkeley, Berkeley, California
Division of Infectious Diseases and Immunity. School of Public Health. Berkeley, California
Abstract: Pathogenic spirochetes of the genus Leptospira are a major cause of human zoonotic infectious disease worldwide. After gaining entry through the skin, the organism causes disease by hematogenously disseminating to multiple organs. The mechanism by which it penetrates the mammalian cell barriers to disseminate is not well understood. In this study, we used a low-passage-number isolate of Leptospira interrogans to elucidate the invasive potential of this spirochete. Quantification of bacteria by dark-field microscopy revealed that pathogenic spirochetes were able to translocate through polarized MDCK cell monolayers at a rate significantly greater than that of nonpathogenic Leptospira or a recognized invasive bacterial pathogen, Salmonella. In contrast to Salmonella, L. interrogans did not alter transepithelial electrical resistance during cell translocation. Both transmission and scanning electron microscopy revealed tight association of the extracellular spirochetes with the host cell plasma membrane, without membrane perturbations suggestive of cytoskeletal rearrangement. Spirochetes were not observed within intercellular junctions or membrane-bound compartments inside cells. They were found within the cytoplasm of only 8% of the counted cells. These results indicate that Leptospira is an invasive but not a facultative intracellular organism. We propose that the rapid translocation of mammalian cells by pathogenic Leptospira is a mechanism designed to evade killing by host cells that permits the organism to quickly reach the bloodstream and disseminate to multiple organs.
DeCS: Polaridade Celular
Células Epiteliais/microbiologia
Leptospira interrogans/patogenicidade
Animais
Linhagem Celular
Cães
Condutividade Elétrica
Células Epiteliais/ultraestrutura
Interações Hospedeiro-Parasita
Junções Intercelulares/ultraestrutura
Rim/citologia
Rim/microbiologia
Microscopia Eletrônica de Varredura
Microvilosidades/ultraestrutura
Fatores de Tempo
Virulência
Issue Date: 2002
Publisher: American Society for Microbiology
Citation: BAROCCHI, M. A. et al. Rapid translocation of polarized MDCK cell monolayers by Leptospira interrogans, an invasive but nonintracellular pathogen. Infection and Immunity, v. 70, n. 12, p. 6926-6932, 2002.
DOI: 10.1128/IAI.70.12.6926–6932.2002
ISSN: 0019-9567
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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