Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7567
Title: Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
Authors: Barocchi, Michele A
Ko, Albert Icksang
Ferrer, Suzana Ramos
Faria, Marcos Tucunduva de
Reis, Mitermayer Galvão dos
Riley, Lee Woodland
Affilliation: Division of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Diseases. Weil Medical College of Cornell University. New York, New York
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Division of Infectious Diseases and Immunity. School of Public Health. University of California. Berkeley, California
Abstract: A new repetitive DNA element was identified in an isolate of Leptospira interrogans serovar copenhageni from a patient in Salvador, Brazil. A Sau3A genomic library from this strain was constructed and screened for repetitive DNA elements. An insert of 438 bp (Rep1) from one library clone hybridized to multiple chromosomal DNA fragments resolved electrophoretically after digestion with BamHI, HindIII, and MfeI. A single oligonucleotide primer, designated iRepl, was designed to generate multiple PCR amplicons of various electrophoretic mobilities in a PCR typing method. The method distinguished strains belonging to the eight pathogenic and three saprophytic species of the genus Leptospira. Clinical isolates obtained during urban epidemics between 1996 and 1998 in Salvador, Brazil, were analyzed by this PCR method. Although the iRep1 primer was unable to discriminate strains among L. interrogans serovar copenhageni isolates, it was able to differentiate strains belonging to different species and serogroups of Leptospira identified in Salvador. This PCR-based method may provide a faster and less expensive alternative to serologic tests used in reference laboratories.
DeCS: Leptospira interrogans/classificação
Leptospira interrogans/genética
Leptospira/classificação
Repetições de Trinucleotídeos/genética
Doença de Weil/microbiologia
Animais
Sequência de Bases
DNA Bacteriano/análise
DNA Bacteriano/genética
Dosagem de Genes
Biblioteca Genômica
Humanos
Leptospira/genética
Leptospira interrogans/isolamento & purificação
Dados de Sequência Molecular
Reação em Cadeia da Polimerase/métodos
Análise de Sequência de DNA
Sorotipagem
Doença de Weil/epidemiologia
Issue Date: 2001
Publisher: American Society for Microbiology
Citation: BAROCCHI, M. A. et al. Identification of new repetitive element in Leptospira interrogans serovar copenhageni and its application to PCR-based differentiation of Leptospira serogroups.
DOI: 10.1128/JCM.39.1.191–195.2001
ISSN: 0095-1137
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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