Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7569
Title: Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis.
Authors: Flannery, Brendan
Costa, Dirceu Joaquim
Carvalho, Fernanda Pinheiro
Guerreiro, Hygia
Matsunaga, James
Silva, Emilson Domingos da
Ferreira, Antonio Gomes Pinto
Riley, Lee Woodland
Reis, Mitermayer Galvão dos
Haake, David A
Ko, Albert Icksang
Affilliation: School of Public Health. University of California. Berkeley, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / School of Pharmacy. Federal University of Bahia. Salvador, BA, Brasil
Division of Infectious Diseases. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California / Department of Medicine. UCLA School of Medicine. Los Angeles, California
Oswaldo Cruz Foundation. Biomanguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil
Oswaldo Cruz Foundation. Biomanguinhos. Brazilian Ministry of Health. Rio de Janeiro, RJ, Brasil
School of Public Health. University of California. Berkeley, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Division of Infectious Diseases. Veterans Affairs Greater Los Angeles Healthcare System. Los Angeles, California / Department of Medicine. UCLA School of Medicine. Los Angeles, California
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. New York, New York
Abstract: There is an urgent need for development of new serodiagnostic strategies for leptospirosis, an emerging zoonosis with worldwide distribution. We have evaluated the diagnostic utility of five recombinant antigens in enzyme-linked immunosorbent assays (ELISAs) for serodiagnosis of leptospirosis. Sera from 50 healthy residents of a high-incidence region were used to determine cutoff values for 96% specificity. In paired sera from 50 cases of leptospirosis confirmed by the microscopic agglutination test, immunoglobulin G (IgG) but not IgM reacted with the recombinant leptospiral proteins. The recombinant LipL32 IgG ELISA had the highest sensitivities in the acute (56%) and convalescent (94%) phases of leptospirosis. ELISAs based on recombinant OmpL1, LipL41, and Hsp58 had sensitivities of 16, 24, and 18% during the acute phase and 72, 44, and 32% during convalescence, respectively. Compared to sera from healthy individuals, patient sera did not react significantly with recombinant LipL36 (P > 0.05). Recombinant LipL32 IgG ELISA demonstrated 95% specificity among 100 healthy individuals, and specificities ranging from 90 to 97% among 30 dengue patients, 30 hepatitis patients, and 16 patients with diseases initially thought to be leptospirosis. Among 39 Venereal Disease Research Laboratory test-positive individuals and 30 Lyme disease patients, 13 and 23% of sera, respectively, reacted positively with the rLipL32 antigen. These findings indicate that rLipL32 may be an useful antigen for the serodiagnosis of leptospirosis.
DeCS: Anticorpos Antibacterianos/sangue
Antígenos de Bactérias/imunologia
Ensaio de Imunoadsorção Enzimática/métodos
Leptospira/imunologia
Leptospirose/diagnóstico
Antígenos de Bactérias/genética
Ensaio de Imunoadsorção Enzimática/normas
Humanos
Imunoglobulina G/sangue
Lipoproteínas/genética
Lipoproteínas/imunologia
Proteínas Recombinantes/genética
Sensibilidade e Especificidade
Issue Date: 2001
Publisher: American Society for Microbiology
Citation: FLANNERY, B. et al. Evaluation of recombinant Leptospira antigen-based enzyme-linked immunosorbent assays for the serodiagnosis of leptospirosis. Journal of Clinical Microbiology, v. 39, n. 9, p. 3303-3310, 2001 .
DOI: 10.1128/JCM.39.9.3303–3310.2001
ISSN: 0095-1137
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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