Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7593
Title: Trypanosoma cruzi gene expression in response to gamma radiation
Authors: Grynberg, Priscila
Silva, Danielle Gomes Passos
Mourão, Marina de Moraes
Hirata Junior, Roberto
Macedo, Andrea Mara
Machado, Carlos Renato
Bartholomeu, Daniella Castanheira
Franco, Glória Regina
Affilliation: Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais Belo Horizonte, MG, Brazil
Universidade de São Paulo. Instituto de Matemática e Estatística. São Paulo, SP, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
Abstract: Trypanosoma cruzi is an organism highly resistant to ionizing radiation. Following a dose of 500 Gy of gamma radiation, the fragmented genomic DNA is gradually reconstructed and the pattern of chromosomal bands is restored in less than 48 hours. Cell growth arrests after irradiation but, while DNA is completely fragmented, RNA maintains its integrity. In this work we compared the transcriptional profiles of irradiated and non-irradiated epimastigotes at different time points after irradiation using microarray. In total, 273 genes were differentially expressed; from these, 160 were up-regulated and 113 down-regulated. We found that genes with predicted functions are the most prevalent in the down-regulated gene category. Translation and protein metabolic processes, as well as generation of precursor of metabolites and energy pathways were affected. In contrast, the up-regulated category was mainly composed of obsolete sequences (which included some genes of the kinetoplast DNA), genes coding for hypothetical proteins, and Retrotransposon Hot Spot genes. Finally, the tyrosyl-DNA phosphodiesterase 1, a gene involved in double-strand DNA break repair process, was up-regulated. Our study demonstrated the peculiar response to ionizing radiation, raising questions about how this organism changes its gene expression to manage such a harmful stress.
Keywords: DNA repair
Gamma radiation Gene expression
Genomic databases Ionizing radiation
Microarrays RNA extraction
Trypanosoma cruzi
Issue Date: 2012
Publisher: Public Library Of Science
Citation: GRYNBERG, Priscila et al. Trypanosoma cruzi gene expression in response to gamma radiation. Plos One. 2012, vol.7, pp. e29596.
DOI: 10.1371/journal.pone.0029596
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos

Files in This Item:
File Description SizeFormat 
Trypanosoma cruzi gene expression in response to gamma radiation.pdf1.79 MBAdobe PDFThumbnail
View/Open



FacebookTwitterDeliciousLinkedInGoogle BookmarksBibTex Format mendeley Endnote DiggMySpace

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.