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https://www.arca.fiocruz.br/handle/icict/7604
EVALUATION OF ENAMEL BY SCANNING ELECTRON MICROSCOPY GREEN LED ASSOCIATED TO HYDROGEN PEROXIDE 35% FOR DENTAL BLEACHING
Author
Affilliation
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Department of Biology. Estate University of Feira de Santana. Feira de Santana, BA, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Biologia Parasitária. Salvador, BA, Brasil
Federal University of Bahia. Center of Biophotonic. School of Dentistry. Salvador, BA, Brasil / National Institute of Optics and Photonics. São Carlos, SP, Brasil / Brugnera & Zanin Institute. São Paulo, SP, Brasil
Abstract
Dental bleaching is a frequently requested procedure in clinical dental practice. The literature is contradictory regarding
the effects of bleaching agents on both morphology and demineralization of enamel after bleaching. The aim of this
study was to analyze by SEM the effect of 35% neutral hydrogen peroxide cured by green LED. Buccal surfaces of 15
pre-molars were sectioned and marked with a central groove to allow experimental and control groups on the same
specimen. For SEM, 75 electron micrographs were evaluated by tree observers at 43X, 220X and 1000X. Quantitative
analysis for the determination of the surface elemental composition of the samples through X-ray microanalysis by SEM
was also performed. The protocol tested neither showed significant changes in mineral composition of the samples nor to
dental enamel structure when compared to controls. SEM analysis allowed inferring that there were marked
morphological differences between the enamel samples highlighting the need for the use of the same tooth in
comparative morphological studies. The tested protocol did not cause morphological damage the enamel surface when
compared to their respective controls.
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