Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7660
Title: Is there a relationship between the detection of human herpesvirus 8 and Epstein-Barr virus in Waldeyer's ring tissues?
Authors: Chagas, Cristiano Aparecido
Endo, Luiza Hayashi
dosSantos, Washington Luis Conrado
Pinto, Glauce Aparecida
Sakano, Eulália
Brousset, Pierre
Vassallo, José
Affilliation: State University of Campinas (UNICAMP). Laboratory of Experimental Pathology. Centro de Assistência Integral à Saúde da Mulher. São Paulo, SP, Brasil
Faculty of Medical Sciences. Department of Otorhinolaryngology. UNICAMP. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
State University of Campinas (UNICAMP). Laboratory of Experimental Pathology. Centro de Assistência Integral à Saúde da Mulher. São Paulo, SP, Brasil
Faculty of Medical Sciences. Department of Otorhinolaryngology. UNICAMP. São Paulo, SP, Brasil
University of Toulouse. Laboratory of Pathology. Toulouse, France
Faculty of Medical Sciences, UNICAMP. Department of Anatomical Pathology. Campinas, SP, Brasil
Abstract: OBJECTIVE: Human herpesvirus 8 (HHV-8) and Epstein-Barr virus (EBV) are human pathogens associated to a number of neoplasms, including tumors of the Waldeyer's ring. Both viruses have been previously detected by in situ methods in tonsils and adenoids from children. HHV-8 was found in 6.8% of the cases and EBV in about one third of the cases. As they belong to the same gamma-herpesvirus subfamily and share some biological characteristics, it is of medical interest to further explore their possible relationship in the Waldeyer's ring, an issue not yet addressed in the specialized literature. The purpose of the present study is to compare the presence of EBV by in situ hybridization (ISH) in tonsils and adenoids from children up to 14 years of age in cases previously shown to be positive and negative for HHV-8. METHODS: Paraffin wax-embedded sections consisting of 38 tonsils and two adenoids from 40 patients were analyzed. HHV-8 was detected by ISH, using the T1-1 probe for the viral mRNA. EBV was also detected by ISH, using the EBER probe. Both probes and the detection systems were provided by Novocastra. RESULTS: HHV-8 was detected in 19 tonsils and one adenoid. The other 19 tonsils and one adenoid taken from the HHV-8-negative group were selected by pairing age and gender of patients with the HHV-8-positive group. In both groups EBV was detected in 13 cases and was negative in other 7. CONCLUSION: Although both viruses are related in many aspects, some biological and epidemiological features differ. This is reflected in the present results, as EBV is similarly detected in the groups negative and positive for HHV-8, favoring different mechanisms of spread.
Keywords: Human herpesvirus 8
Epstein—Barr virus
Tonsils
Adenoids
In situ hybridization
DeCS: Tonsila Faríngea/virologia
Herpesvirus Humano 4/isolamento & purificação
Tonsila Palatina/virologia
Tonsila Faríngea/química
Adolescente
Criança
Pré-Escolar
DNA Viral/análise
Feminino
Humanos
Hibridização In Situ
Lactente
Masculino
Tonsila Palatina/química
Inclusão em Parafina
Issue Date: 2006
Publisher: Elsevier
Citation: CHAGAS, C. A. et al. Is there a relationship between the detection of human herpesvirus 8 and Epstein-Barr virus in Waldeyer's ring tissues? International Journal of Pediatric Otorhinolaryngology, v. 70, p. 1923—1927, 2006.
DOI: 10.1016/j.ijporl.2006.06.020
ISSN: 0165-5876
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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