Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7661
Title: Role of interleukin-4 and prostaglandin E2 in Leishmania amazonensis infection of BALB/c mice.
Authors: Guimarães, Elisalva Teixeira
Santos, Luana A
Santos, Ricardo Ribeiro dos
Teixeira, Mauro Martins
dosSantos, Washington Luis Conrado
Soares, Milena Botelho Pereira
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Abstract: The role of cytokines in Leishmania amazonensis experimental infection has not been as well studied as in Leishmania major infection model. Here we investigated the role of interleukin (IL)-4 and PGE(2) in L. amazonensis infection of susceptible BALB/c mice. IL-4 deficient (-/-) or wild-type (+/+) BALB/c mice were infected with different inocula of L. amazonensis. Two weeks after infection with 5x10(6) promastigotes/footpad, the production of interferon (IFN)-gamma upon L. amazonensis antigen stimulation was significantly higher in lymph node cell cultures of IL-4-/- mice than in IL-4+/+ mice. The levels of anti-leishmania IgG2a antibodies were also significantly higher in serum from IL-4-/- mice. In contrast, the levels of IgG1 antibodies were increased in IL-4+/+ mice and almost undetectable in IL-4-/- mice. Despite the increased Th1 response, lesions of IL-4-/- BALB/c mice progressed similarly to those of IL-4+/+ mice upon infection with the 5x10(6) inoculum. However, IL-4-/- mice developed smaller lesions upon infection with 10(5), 10(4) or 10(3) parasites than IL-4+/+ mice. The resistance of IL-4-/- correlated with higher Th1 response, compared to IL-4+/+ upon infection with 10(4)L. amazonensis. IL-4+/+ mice treated with indomethacin, an inhibitor of PGE(2) synthesis, during the first 3weeks of infection developed smaller lesions and lower parasitic load when compared to the control group. The lesions of indomethacin-treated groups contained mostly macrophages without vacuoles and small or absent necrotic areas. These results indicate that IL-4 and PGE(2) are susceptibility factors to L. amazonensis infection.
Keywords: Leishmania amazonensis
BALB/c mice
Interleukin-4
Prostaglandin E2
Indomethacin
DeCS: Dinoprostona/metabolismo
Interleucina-4/metabolismo
Leishmania mexicana
Leishmaniose Cutânea/imunologia
Animais
Anti-Inflamatórios não Esteroides/uso terapêutico
Suscetibilidade a Doenças
Feminino
Humanos
Indometacina/uso terapêutico
Interferon gama/biossíntese
Leishmania mexicana/imunologia
Leishmaniose Cutânea/quimioterapia
Leishmaniose Cutânea/fisiopatologia
Camundongos
Camundongos Endogâmicos BALB C
Issue Date: 2006
Publisher: Elsevier
Citation: GUIMARÃES, E. T. et al. Role of interleukin-4 and prostaglandin E2 in Leishmania amazonensis infection of BALB/c mice. Microbes and Infection, v. 8, n. 5, p. 1219-1226, 2006.
DOI: 10.1016/j.micinf.2005.11.011
ISSN: 1286-4579
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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