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AutorProbst, Christian M.
AutorSilva, Rodrigo de Araújo
AutorMenezes, Juliana Perrone Bezerra de
AutorAlmeida, Taís Fontoura de
AutorGomes, Ivana Nunes
AutorDallabona, Andréia C.
AutorOzaqui, L. S.
AutorBuck, Gregory A.
AutorPavoni, Daniela P.
AutorKrieger, Marco Aurélio
AutorVeras, Patrícia Sampaio Tavares
Fecha de acceso2014-05-29T19:54:51Z
Fecha de disponibilización2014-05-29T19:54:51Z
Fecha de publicación2012
ReferenciaPROBST, C. M. et al. A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection. BMC Microbiology, v. 12, p. 22, 2012.pt_BR
ISSN1471-2180
URIhttps://www.arca.fiocruz.br/handle/icict/7727
Idiomaengpt_BR
EditorBioMed Centralpt_BR
Derechos de autoropen accesspt_BR
TítuloA comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.pt_BR
Tipo del documentoArticlept_BR
Resumen en InglésBACKGROUND: The experimental murine model of leishmaniasis has been widely used to characterize the immune response against Leishmania. CBA mice develop severe lesions, while C57BL/6 present small chronic lesions under L. amazonensis infection. Employing a transcriptomic approach combined with biological network analysis, the gene expression profiles of C57BL/6 and CBA macrophages, before and after L. amazonensis infection in vitro, were compared. These strains were selected due to their different degrees of susceptibility to this parasite. RESULTS: The genes expressed by C57BL/6 and CBA macrophages, before and after infection, differ greatly, both with respect to absolute number as well as cell function. Uninfected C57BL/6 macrophages express genes involved in the deactivation pathway of macrophages at lower levels, while genes related to the activation of the host immune inflammatory response, including apoptosis and phagocytosis, have elevated expression levels. Several genes that participate in the apoptosis process were also observed to be up-regulated in C57BL/6 macrophages infected with L. amazonensis, which is very likely related to the capacity of these cells to control parasite infection. By contrast, genes involved in lipid metabolism were found to be up-regulated in CBA macrophages in response to infection, which supports the notion that L. amazonensis probably modulates parasitophorous vacuoles in order to survive and multiply in host cells. CONCLUSION: The transcriptomic profiles of C57BL/6 macrophages, before and after infection, were shown to be involved in the macrophage pathway of activation, which may aid in the control of L. amazonensis infection, in contrast to the profiles of CBA cells.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, Brasilpt_BR
AfiliaciónCenter for the Study of Biological Complexity. Virginia Commonwealth University. Richmond, VA, USApt_BR
AfiliaciónCenter for the Study of Biological Complexity. Virginia Commonwealth University. Richmond, VA, USApt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, Brasilpt_BR
AfiliaciónFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasilpt_BR
DeCSPerfilação da Expressão Gênicapt_BR
DeCSInterações Hospedeiro-Patógenopt_BR
DeCSLeishmania mexicana/imunologiapt_BR
DeCSLeishmania mexicana/patogenicidadept_BR
DeCSMacrófagos/imunologiapt_BR
DeCSMacrófagos/parasitologiapt_BR
DeCSAnimaispt_BR
DeCSFemininopt_BR
DeCSCélulas Cultivadaspt_BR
DeCSMasculinopt_BR
DeCSCamundongos Endogâmicos C57BLpt_BR
DeCSCamundongospt_BR
DeCSAnálise em Microssériespt_BR
DeCSReação em Cadeia da Polimerase em Tempo Realpt_BR


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