Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7751
Title: Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
Authors: Gomes, Ivana Nunes
Calabrich, Aknar Freire de Carvalho
Tavares, Rafael da Silva
Wietzerbin, Jeanne
Freitas, Luiz Antonio Rodrigues de
Veras, Patrícia Sampaio Tavares
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina da Universidade Federal da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil.
Unité Inserm U365. Institut Curie. Paris, France.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina da Universidade Federal da Bahia. Salvador, BA, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.
Abstract: While CBA/J mice fail to be permissive to Leishmania amazonensis-driven pathogenic processes, they heal easily following Leishmania major infection. The early-phase events are crucial to the outcome of Leishmania infection and it is known that macrophages (Mφ) are important in infection control. In the present study we investigated the role of Mφ in driving CBA/J susceptibility to L. amazonensis. We performed kinetic studies and compared the capacity of L. amazonensis and L. major to infect Mφ. There was no difference in percentages of infection or parasite burden for 6 h between the two groups. In contrast, after 12 h we observed that infection was about twice as high in L. amazonensis- than in L. major-infectedMφ. In addition, rIFN-c added to the cultures induced nitric oxide (NO) production, and did not modify L. amazonensis infection, although the percentage of L. major infection was significantly reduced. This reduction in L. major infection is a TNF-a dependent mechanism as L. major-infected Mφ expressed twice as much TNF-a mRNA as L. amazonensis-infected cells, and anti-TNF-a reversed the IFN-c effect. Moreover, rTNF-a plus IFN-c were able to significantly reduce the percentage of L. amazonensisinfected cells but not to the same extent as in L. major infection. Despite having higher NO production than IFN-c-treated cells,AMGaddition to IFN-c-plus TNF-a-treated cells only partially reversed the inhibition in L. major, but not in L. amazonensis infection. Thus, in this study, we demonstrated that L. amazonensis both inactivated and resisted innate and IFN-c-induced Mφ killing mechanisms, indicating that the nature of the parasite and its interaction with Mφ could determine immune response polarization.
Keywords: Macrophages
CBA/J mice
L. amazonensis
DeCS: Leishmaniose/imunologia
Macrófagos/imunologia
Macrófagos/parasitologia
Células Cultivadas
Animais
Feminino
Peróxido de Hidrogênio/metabolismo
Inflamação/imunologia
Interferon gama/metabolismo
Interleucina-10/farmacologia
Leishmania/classificação
Leishmania/efeitos de drogas
Leishmania/patogenicidade
Leishmaniose/parasitologia
Leishmaniose/patologia
Leishmaniose Cutânea/patologia
Masculino
Camundongos
Camundongos Endogâmicos CBA
Especificidade da Espécie
Fator de Crescimento Transformador beta/farmacologia
Fator de Necrose Tumoral alfa/fisiologia
Issue Date: 2003
Publisher: Elsevier
Citation: GOMES, I. N. et al. Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania. Microbes and Infection, v. 5, n. 4, p. 251-260, 2003.
DOI: 10.1016/S1286-4579(03)00025-X
ISSN: 1286-4579
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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