Author | Silva, Tânia Regina Marques da | |
Author | Freitas, Juliana Ribeiro de | |
Author | Silva, Queilan Chagas | |
Author | Figueira, Cláudio Pereira | |
Author | Roxo, Eliana | |
Author | Leão, Sylvia Cardoso | |
Author | Freitas, Luiz Antonio Rodrigues de | |
Author | Veras, Patrícia Sampaio Tavares | |
Access date | 2014-06-02T18:39:25Z | |
Available date | 2014-06-02T18:39:25Z | |
Document date | 2002 | |
Citation | SILVA, T. et al. Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion. Infection and Immunity, v. 70, n. 10, p. 5628-5634, 2002. | pt_BR |
ISSN | 0019-9567 | |
URI | https://www.arca.fiocruz.br/handle/icict/7752 | |
Language | eng | pt_BR |
Publisher | American Society for Microbiology | pt_BR |
Rights | open access | pt_BR |
Title | Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion. | pt_BR |
Type | Article | pt_BR |
DOI | 10.1128/IAI.70.10.5628–5634.2002 | |
Abstract | The virulence of different isolates of Mycobacterium has been associated with two morphologically distinguishable
colonial variants: opaque (SmOp) and transparent (SmTr). In this report we used an in vitro assay
to compare macrophage (M ) responses to SmOp and SmTr Mycobacterium fortuitum variants, taking advantage
of the fact that these variants were derived from the same isolate. Cells preactivated or not with gamma
interferon (IFN- ) were infected with SmOp or SmTr M. fortuitum. We showed that SmOp and SmTr induced
different levels of nitric oxide (NO) production by IFN- -stimulatedM . Indeed, the amount of IFN- -induced
NO production by J774 cells was 4.8 to 9.0 times higher by SmOp (23.1 to 37.7 M) compared to SmTr
infection (3.9 to 4.8 M) (P 0.0332), indicating that virulent SmTr bacilli restricted NO production. In
addition, IFN- -induced NO production by M was higher when correlated with reduction of only avirulent
SmOp bacillus viability. SNAP (S-nitroso-N-acetyl-DL-penicillamine)-induced NO production did not modify
SmTr viability, indicating its resistance to nitrogen radicals. Electron microscopy studies were performed to
evaluate the capacity of phagosomes to fuse with lysosomes labeled with bovine serum albumin-colloidal gold
particles. By 24 h postinfection, 69% more phagosome-containing SmOp variant had fused with lysosomes
compared to the SmTr-induced phagosomes. In conclusion, these data indicate that virulent SmTr bacilli may
escape host defense by restricting IFN- -induced NO production, resisting nitrogen toxic radicals, and limiting
phagosome fusion with lysosomes. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina. Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina. Universidade Federal da Bahiao. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil | pt_BR |
Affilliation | Instituto Biológico. São Paulo, SP, Brasil | pt_BR |
Affilliation | Universidade Federal de São Paulo. UNIFESP/EPM. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina. Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil /Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
DeCS | Interferon gama/farmacologia | pt_BR |
DeCS | Lisossomos/fisiologia | pt_BR |
DeCS | Macrófagos/microbiologia | pt_BR |
DeCS | Macrófagos/fisiologia | pt_BR |
DeCS | Mycobacterium fortuitum/patogenicidade | pt_BR |
DeCS | Óxido Nítrico/biossíntese | pt_BR |
DeCS | Fagossomos/fisiologia | pt_BR |
DeCS | Animais | pt_BR |
DeCS | Linhagem Celular | pt_BR |
DeCS | Variação Genética | pt_BR |
DeCS | Lisossomos/microbiologia | pt_BR |
DeCS | Ativação de Macrófagos/efeitos de drogas | pt_BR |
DeCS | Macrófagos/ultraestrutura | pt_BR |
DeCS | Fusão de Membrana | pt_BR |
DeCS | Camundongos | pt_BR |
DeCS | Microscopia Eletrônica | pt_BR |
DeCS | Mycobacterium fortuitum/isolamento & purificação | pt_BR |
DeCS | Fagossomos/microbiologia | pt_BR |
DeCS | Proteínas Recombinantes | pt_BR |
DeCS | Virulência/genética | pt_BR |