Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7788
Title: CD8+ T cells in situ in different clinical forms of human cutaneous leishmaniasis.
Authors: Dantas, Marina Loyola
Oliveira, Juliana Cabral de
Carvalho, Lucas Pedreira de
Passos, Sara Timóteo
Queiroz, Adriano
Machado, Paulo Roberto
Carvalho Filho, Edgar Marcelino
Arruda, Sérgio Marcos
Affilliation: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Hospital Universitário Professor Edgard Santos. Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais Conselho Nacional de Desenvolvimento Científico e Tecnológico. Salvador, BA, Brasil
Hospital Universitário Professor Edgard Santos. Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais Conselho Nacional de Desenvolvimento Científico e Tecnológico. Salvador, BA, Brasil
Hospital Universitário Professor Edgard Santos. Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais Conselho Nacional de Desenvolvimento Científico e Tecnológico. Salvador, BA, Brasil
Hospital Universitário Professor Edgard Santos. Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais Conselho Nacional de Desenvolvimento Científico e Tecnológico. Salvador, BA, Brasil
Hospital Universitário Professor Edgard Santos. Universidade Federal da Bahia. Serviço de Imunologia. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Doenças Tropicais Conselho Nacional de Desenvolvimento Científico e Tecnológico. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil
Abstract: Introduction: Leishmania braziliensis infection induces a large spectrum of lesions that clinically manifest as nodules or papules that progress to ulcers. Although it is already known that T helper cells predominate in the lesions, cytotoxic T cells have also been reported to be present, and their role in leishmaniasis immunopathogenesis is not well known. This study investigated the amounts of CD8+ and granzyme B+ cells in different clinical forms of human cutaneous leishmaniasis (CL). Methods: Forty tissue fragments from early (E-CL) and late CL (L-CL) lesions and from disseminated leishmaniasis (DL) - papules and ulcers - were characterized. The infl amed area per fragment was calculated, and the CD8 and granzyme B expression levels in the infi ltrates were quantifi ed by counting positive cells in 15 fi elds. The localization of CD8 and granzyme B was graded subjectively. Results: Infl ammation was higher in L-CL and DL ulcers. CD8 expression was increased in late ulcerated lesions compared to recent lesions. The increase in CD8+ cells also correlated with the duration of the lesion. Papules had a higher frequency of granzyme B+ cells than E-CL lesions, although the frequency was similar to those for late and DL ulcers. CD8+ cells were mostly found in the papillary dermis. Conclusions: CD8+ T and granzyme B+ cells are present in the infl ammatory infi ltrates of CL and DL and may participate in the immunopathogenesis of Leishmania infection.
Keywords: Leishmaniasis
Cytotoxicity
Infl ammation
Cutaneous.
CD8
Human
Linfócitos T CD8-Positivos/enzimologia
DeCS: Linfócitos T CD8-Positivos/imunologia
Granzimas/imunologia
Leishmaniose Cutânea/imunologia
Adolescente
Adulto
Criança
Feminino
Humanos
Imuno-Histoquímica
Leishmaniose Cutânea/patologia
Masculino
Meia-Idade
Adulto Jovem
Issue Date: 2013
Publisher: Sociedade Brasileira de Medicina Tropical
Citation: DANTAS, M. L. et al. CD8+ T cells in situ in different clinical forms of human cutaneous leishmaniasis. Revista da Sociedade Brasileira de Medicina Tropical, v. 46, n. 6, p. 728-734, 2013.
DOI: dx.doi.org/10.1590/0037-8682-0174-2013
ISSN: 1678-9849
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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