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https://www.arca.fiocruz.br/handle/icict/7818
P16(INK4A) EXPRESSION AS A POTENTIAL PROGNOSTIC MARKER IN CERVICAL PRE-NEOPLASTIC AND NEOPLASTIC LESIONS.
Diagnostic marker
p16INK4a
Human papillomavirus; Human immunodeficiency virus
Inibidor p16 de Quinase Ciclina-Dependente/metabolismo
Lesões Pré-Cancerosas/diagnóstico
Marcadores Biológicos de Tumor/análise
Neoplasias do Colo do Útero/diagnóstico
Carcinoma/diagnóstico
Neoplasia Intraepitelial Cervical/complicações
Feminino
Colo do Útero/metabolismo
Infecções por HIV/complicações
Infecções por HIV/diagnóstico
Humanos
Imuno-Histoquímica
Infecções por Papillomavirus/complicações
Infecções por Papillomavirus/diagnóstico
Prognóstico
Author
Affilliation
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University São Paulo. Department of Pathology. Adolfo Lutz Institute. São Paulo, SP, Brasil
Virology Department. Ludwig Institute for Cancer Research. Sao Paulo, SP, Brasil
Virology Department. Ludwig Institute for Cancer Research. Sao Paulo, SP, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University São Paulo. Department of Pathology. Adolfo Lutz Institute. São Paulo, SP, Brasil
Virology Department. Ludwig Institute for Cancer Research. Sao Paulo, SP, Brasil
Virology Department. Ludwig Institute for Cancer Research. Sao Paulo, SP, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Federal University of Bahia. Department of Gynecology and Obstetrics Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Federal University of Bahia. Department of Pathology. Medical School, and Mathematics and Statistic School. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
Abstract
An immunohistochemical analysis with monoclonal antibody p16INK4a was performed in formalin-fixed, paraffinembedded
samples of 60 cases. The aim was to investigate in biopsies the expression of p16INK4a of normal uterine
cervical tissue, pre-cancerous and cancerous lesions, and their relation with human papilloma virus (HPV) and HIV
status. Three parameters were evaluated: percentage of p16INK4a positive cells, reaction intensity, and cell staining
pattern. All of these parameters were statistically different when compared among different histological groups.
However, logistic regression model showed that the reaction intensity was the best indicator of the expression of
p16INK4a. This expression increases from normal to invasive squamous carcinoma. Sixty-six percent of the patients
with CIN grade 1 (CIN1) expressed p16INK4a (all these cases were infected with high risk HPV). Our study supports the
hypothesis that p16INK4a expression in pre-cancerous lesions and cancers can be used to identify HPV-transformed
cells. Of great interest for routine diagnostic use is the fact that immunohistochemical testing for p16INK4a seems to be
capable of identifying HPV-positive cells and potentially recognizing those lesions with an increased risk of progression
to high-grade lesions
Keywords
Cervical neoplasiaDiagnostic marker
p16INK4a
Human papillomavirus; Human immunodeficiency virus
DeCS
Neoplasia Intraepitelial Cervical/diagnósticoInibidor p16 de Quinase Ciclina-Dependente/metabolismo
Lesões Pré-Cancerosas/diagnóstico
Marcadores Biológicos de Tumor/análise
Neoplasias do Colo do Útero/diagnóstico
Carcinoma/diagnóstico
Neoplasia Intraepitelial Cervical/complicações
Feminino
Colo do Útero/metabolismo
Infecções por HIV/complicações
Infecções por HIV/diagnóstico
Humanos
Imuno-Histoquímica
Infecções por Papillomavirus/complicações
Infecções por Papillomavirus/diagnóstico
Prognóstico
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