Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7829
Title: CD4-CD8-αβ and γδ T cells display inflammatory and regulatory potentials during human tuberculosis
Authors: Pinheiro, Melina de Barros
Antonelli, Lis Ribeiro do Valle
Avelar, Renato Sathler
Avelar, Danielle Marquete Vitelli
Miranda, Silvana Spindola de
Guimarães, Tânia Mara Pinto Dabés
Carvalho, Andréa Teixeira de
Martins Filho, Olindo Assis
Toledo, Vicente de Paulo Coelho Peixoto de
Affilliation: Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Departamento de Clínica Médica. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil
Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil
Abstract: T-cells play an important role controlling immunity against pathogens and therefore influence the outcome of human diseases. Although most T-lymphocytes co-express either CD4 or CD8, a smaller T-cell subset found the in the human peripheral blood that expresses the αβ or γδ T-cell-receptor (TCR) lacks the CD4 and CD8 co-receptors. These double negative (DN) T-cells have been shown to display important immunological functions in human diseases. To better understand the role of DN T-cells in human Mycobacterium tuberculosis, we have characterized their frequency, activation and cytokine profile in a well-defined group of tuberculosis patients, categorized as severe and non-severe based on their clinical status. Our data showed that whereas high frequency of αβ DN T-cells observed in M. tuberculosis-infected patients are associated with disease severity, decreased proportion of γδ DN T-cells are found in patients with severe tuberculosis. Together with activation of CD4+ and CD8+ T-cells, higher frequencies of both αβ and γδ DN T-cells from tuberculosis patients also express the chronic activation marker HLA-DR. However, the expression of CD69, an early activation marker, is selectively observed in DN T-cells. Interestingly, while αβ and γδ DN T-cells from patients with non-severe tuberculosis display a pro-inflammatory cytokine profile, characterized by enhanced IFN-γ, the γδ DN T-cells from patients with severe disease express a modulatory profile exemplified by enhanced interleukin-10 production. Overall, our findings suggest that αβ and γδ DN T-cell present disparate immunoregulatory potentials and seems to contribute to the development/maintenance of distinct clinical aspects of TB, as part of the complex immunological network triggered by the TB infection.
Keywords: Mycobacterium tuberculosis
T-cells
Issue Date: 2012
Publisher: Public Library of Science one
Citation: PINHEIRO, Melina de Barros et al. CD4-CD8-αβ and γδ T cells display inflammatory and regulatory potentials during human tuberculosis. Plos One. 2012, vol.7, pp. 50923
DOI: 10.1371/journal.pone.0050923
ISSN: 1932-6203
Copyright: open access
Appears in Collections:IRR - Artigos de Periódicos

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