Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/7843
Title: Cell-to-cell transfer of Leishmania amazonensis amastigotes is mediated by immunomodulatory LAMP-rich parasitophorous extrusions
Authors: Real, Fernando
Florentino, Pilar Tavares Veras
Reis, Luiza Campos
Sanchez, Eduardo M. Ramos
Veras, Patrícia Sampaio Tavares
Goto, Hiro
Mortara, Renato Arruda
Affilliation: Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Universidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil
Universidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais (INCT – DT). Salvador, BA, Brasil
Universidade de São Paulo. Instituto de Medicina Tropical. Laboratório de Soroepidemiologia e Imunobiologia. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Medicina Preventiva. São Paulo, SP, Brasil
Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil
Abstract: The last step of Leishmania intracellular life cycle is the egress of amastigotes from the host cell and their uptake by adjacent cells. Using multidimensional live imaging of long-term-infected macrophage cultures we observed that Leishmania amazonensis amastigotes were transferred from cell to cell when the donor host macrophage delivers warning signs of imminent apoptosis. They were extruded from the macrophage within zeiotic structures (membrane blebs, an apoptotic feature) rich in phagolysosomal membrane components. The extrusions containing amastigotes were selectively internalized by vicinal macrophages and the rescued amastigotes remain viable in recipient macrophages. Host cell apoptosis induced by micro-irradiation of infected macrophage nuclei promoted amastigotes extrusion, which were rescued by non-irradiated vicinal macrophages. Using amastigotes isolated from LAMP1/LAMP2 knockout fibroblasts, we observed that the presence of these lysosomal components on amastigotes increases interleukin 10 production. Enclosed within host cell membranes, amastigotes can be transferred from cell to cell without full exposure to the extracellular milieu, what represents an important strategy developed by the parasite to evade host immune system.
keywords: Leishmania
Apoptose
Celula
Interações hospedeiro-parasita
Issue Date: 2014
Publisher: John Wiley & Sons Ltd
Citation: REAL, F. et al. Cell-to-cell transfer of Leishmania amazonensis amastigotes is mediated by immunomodulatory LAMP-rich parasitophorous extrusions. Cellular Microbiology, v.16, n.10, p.1549-1564, 2014.
DOI: 10.1111/cmi.12311
ISSN: 1462-5814
Copyright: open access
Appears in Collections:BA - IGM - Artigos de Periódicos

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