Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/787
Title: Anti-HIV-1 seroreactivity and HIV transmission route[R1]
Authors: Bongertz, Vera
Guimarães, Monick Lindenmeyer
Costa, M. F. G. Soares da
Santos, Valdiléa Gonçalves Veloso dos
Bastos, Francisco Inácio Pinkusfeld Monteiro
Szwarcwald, Celia Landmann
Derrico, Monica
Dias, Paulo Roberto Telles Pires
Pilotto, Jose Henrique da Silva
João Filho, Esaú Custódio
Morgado, Mariza Gonçalves
Affilliation: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro Centro de Informação Cientifica e Tecnológica. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Informação Cientifica e Tecnológica Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Centro de Informação Cientifica e Tecnológica. Rio de Janeiro, RJ, Brasil
Universidade Estadual do Rio de Janeiro. NEPAD. Rio de Janeiro, RJ, Brasil
Hospital Geral de Nova Iguaçu. Rio de Janeiro, RJ, Brasil
Hospital dos Servidores do Estado. Rio de Janeiro, RJ, Brasil
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Hospital Evandro Chagas. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil
Abstract: Background: antibody binding assays carried out by our group have consistently indicated a higher reactivity of sera from male HIV-1 infected individuals. This study was carried out in order to analyze the importance of gender, route of transmission, disease progression and HIV-1 genotype in seroreactivity assays. Study design: specificity of antibody binding was studied in plasma of 247 HIV-1 seropositive individuals belonging to patient groups of pregnant women, injecting drug users (IDUs) and recent seroconvertors, resident in Rio de Janeiro, RJ. Recognition of synthetic peptides corresponding to antigenically important epitopes in the envelope of HIV-1 (gp41 immunodominant epitope, V3 loop, V2 loop and gp41 735–752 epitope) was determined. Results: the immunodominant gp41 peptide (amino acids 594–613, HIV-1 MN sequence) was recognized by 85% of all plasma tested. Reactivity with the gp41 735–752 peptide and gp120 V2 loop peptides was low but quite variable, being generally more often specific to a Brazilian V2 peptide used than to the HIV-1 MN derived V2 peptide. The overall recognition of the different V3 peptides tested varied from 41 to 76%. Patients with more advanced disease showed a more frequent reactivity with the peptides studied than did asymptomatic patients. Statistically significant differences in peptide recognition were observed by multiple logistic analyses comparing plasma derived from individuals infected by blood or sexual HIV transmission, adjusting for disease progression and gender. Plasma from individuals infected by sexual transmission showed lower peptide recognition than did plasma from individuals infected through HIV positive blood. Association attempts between seroreactivity and genotype indicated that plasma derived from patients infected with HIV-1 of the F subtype showed highest recognition of heterologous V3 peptides, as well as a slightly more frequent recognition of the non-V3 peptides tested. Recognition of homologous peptides was generally higher than recognition of heterologous peptides. Differences were most pronounced between the prototypical HIV-1 B subtype and the Brazilian B′′ variant of this subtype but almost non-existent between the HIV-1 B and F subtypes. Conclusions: individual gender was shown to be a confounder when investigating the relationships of peptide reaction to HIV-1 route of transmission through multivariate statistical methods: patients infected by blood transmission (IDU) present higher frequency of peptide recognition than individuals infected by sexual HIV-1 transmission. Plasma from individuals infected with the B′′ variant (GWG) of B subtype HIV-1 showed lower heterologous peptide recognition than that from HIV-1 B (GPG) or F infected individuals.
Keywords: HIV
Seroreactivity
Transmission
Genotypes
Issue Date: 1999
Publisher: Elsevier Science B.V.
Citation: BONGERTZ, V. et al. Anti-HIV-1 seroreactivity and HIV transmission route[R1]. Journal of Clinical Virology, v. 12, p. 27–36, 1999.
ISSN: 1386-6532
Copyright: open access
Appears in Collections:INI - Artigos de Periódicos
ICICT - Artigos de Periódicos

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