Please use this identifier to cite or link to this item: https://www.arca.fiocruz.br/handle/icict/8066
Title: Characterization of bovine transcripts preferentially expressed in testis and with a putative role in spermatogenesis
Authors: Reis, Beatriz da Costa Aguiar Alves
Tobo, Patrícia Renovato
Rodrigues, Rúbia
Ruiz, Jerônimo Conceição
Lima, Vera Fernanda Martins Hossepian de
Moreira Filho, Carlos Alberto
Affilliation: Universidade de São Paulo. Centro de Pesquisas em Biotecnologia. São Paulo, SP, Brazil
Universidade de São Paulo. Centro de Pesquisas em Biotecnologia. São Paulo, SP, Brazil
Universidade de São Paulo. Centro de Pesquisas em Biotecnologia. São Paulo, SP, Brazil
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil
Faculdade de Ciências Agrárias e Veterinárias. Departamento de Reprodução Animal. Universidade Estadual Paulista. Jaboticabal, SP, Brazil
Universidade de São Paulo. Faculdade de Medicina. Departamento de Pediatria. São Paulo, SP, Brazil
Abstract: Although the number of genes known to be associated with bovine spermatogenesis has increased in the past few years, regulation of this biological process remains poorly understood. Therefore, discovery of new male fertility genetic markers is of great value for assisted selection in commercially important cattle breeds, e.g., Nelore, that have delayed reproductive maturation and low fertility rates. The objective of the present study was to identify sequences associated with spermatogenesis that could be used as fertility markers. With RT-PCR, the following five transcripts preferentially expressed in adult testis were detected: TET656 detected only in adult testis; TET868 and TET515 expressed preferentially in adult testis but also detected in fetal gonads of both sexes; and TET456 and TET262, expressed primarily in the testis, but also present in very low amounts in somatic tissues. Based on their homologies and expression profiles, we inferred that they had putative roles in spermatogenesis. Detection of sequences differentially expressed in testis, ovary, or both, was a useful approach for identifying new genes related to bovine spermatogenesis. The data reported here contributed to discovery of gene pathways involved in bovine spermatogenesis, with potential for prediction of fertility.
Keywords: Spermatogenesis
Testis gene expression
Fertility marker
Differential gene expression
qPCR
Issue Date: 2011
Publisher: Elsevier Inc.
Citation: ALVES, Beatriz da Costa Aguiar et al. Characterization of bovine transcripts preferentially expressed in testis and with a putative role in spermatogenesis. Theriogenology, 76(6): 991-998. 2011.
DOI: 10.1016/j.theriogenology.2011.04.027
ISSN: 0093691X
Copyright: open access
Appears in Collections:MG - IRR - Artigos de Periódicos




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